非編碼基因zm401對(duì)玉米小孢子發(fā)育的影響.pdf

1、丫773562分類號(hào)：密緞：飛嚼差專夭雜。博士學(xué)位論文單位代碼：10019學(xué)號(hào)；B02269非編碼基因zm401對(duì)玉米小孢子發(fā)育的影響TheEffectofalion—codingRNAGenezm401onMicrosporeDevelopmentinMaize研究生：呈壘霞指導(dǎo)教師：教堂盟熬攫申請(qǐng)學(xué)位門類級(jí)別：堡堂墟專業(yè)名稱：生物焦堂皇筮王生麴堂研究方向：揎物基固王捏所在學(xué)院：生塹堂睦2005年6月AbstractMalerepro

2、ductiveprocessesinfloweringplantstakeplaceinthestamenThemalegametophyte(pollengrain)offloweringplantsistheproducerandthecarderofgametesInasingleuniteachgraincontainsallthegeneticinformationrequiredtounitewiththefemalegam

3、eteatfertilizationandtoformanewsporophyteThedevelopmentaleventsleadingtoantherformationandpollenreleaseareexquisitelytimedandchoreographedTranscriptionalactivationofspecificgenesetsisrequiredforthereestablishmentandmaint

4、enanceofdifferentiatedcelltypesandfunctionsduringantherdevelopmentThebegainingandmatureofmalegametophyteoffloweringplantsarecontrolledbyaseriesofgenesinthemalestamenGenesregularmeiosisofpollenmothercells，microspomforming

5、stageandtheformingprocessofthemalegametophyteBecauseofcrucialfunctionintheplant’sreproductivecycle，pollenistheobjectofbiochemicalandmolecularbiologicalresearchIdentifyinggenesthatareresponsiblefurpollendevelopmentisveryi

6、mportantInourpreviousstudyaeDNAlibrarytopoly(A)RNAisolatedfrommaturepollenofzeamayswasconstructedDifferentialscreeningandColdplaquescreeningobtainedoneeDNAfragment；designatedzm401(Zeamays)，fulllengthofzm401eDNAwasgenerat

7、edby5’and3’RACEmethodzm401eDNAwas1149bpinlengthzm401wasspecificallyorpreferentiallyexpressedinmaturepollenTheanalysisresultsbybiologicsoftwaresuggestthatzm401wasoneofagrowingnumberofnon—codingmRNA—likeRNAtranscriptsthate

8、xerttheircellularfunctionsdirectlyasRNAWeperformedinvitrotranslationtofurtherstudyifzm401couldbetranslatedinvitroTheresultthatzm401eDNAcouldnotbetranslatedconfirmedtheconclusionthatzm401couldbeamemberofanewcategoryofnonc

9、odingRNAButwecouldnotknowifzm401canbetranslatedinvivoorexecuteitsfunctionbyRNAformTheexactfunctionsofzm401remaintobeelucidated，andfurtheranalysisofzm401mayprovideawaytoelucidatethemechanismofantherdevelopmentTogainmorein

10、formationaboutthefunctionofzm40lgene，plantexpressionvectorswithzm401RNAiconstructandsensezm40lgenepromotedbyZMl3promoterwereconstructedMaizeembryogeniccallusWeretransformedbymicroprojectilebombardmentwiththeseplantexpres

11、sionvectorsAfterselectingforhygromycinresistance，10plantswithzm401RNAiconstructand6plantswithsensezm401respectivelywereobtainedbygenomicPCR，southernblottinganalysisTheaberrantphenotypeswereobservedintheantherofthetransge

12、nicplantswjtllzm401RNAiconstructandsensezm401geneThetasselsoftransgenicplantswithzm401RNAiconstructcouldnotopenwhenthetasselsofcontrolplantsdid，andthetoptasselsoftransgenicplantswithsensezm40lgeneweredysplasiashriveledan

13、dwitheredHistologicaldifferenceswereobservablefortransgenicplantsComparingthenon。transformantwithtransgenicplantmicrosporesinthenontransformingwereroundednormally；theshapeofmostofthepollengrainsoftransgenicplantsbecamedi