球毛殼菌EST分析及生物防治相關(guān)基因生物信息學(xué)研究.pdf

1、哈爾濱工業(yè)大學(xué)碩士學(xué)位論文球毛殼菌EST分析及生物防治相關(guān)基因生物信息學(xué)研究姓名：李巖松申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：人機(jī)與環(huán)境工程指導(dǎo)教師：楊謙20050601鑒璽堡：：些鑾蘭三蘭鎏圭蘭堡蘭鑾AbstractChaetomiumglobosumbelongstoAscomycutaisanexcellentbio—controlmicrobeformanyplantdiseaseswhichhastheeffectofenecationon

2、pathogenicbacteriaofplantbyvariousmechanismTheresearchofglobosummyceliaExpressedSequenceTagsprovidesimportanttheoreticalandexperimentfoundationforbiologymechanismresearchonthelevelofmoleculeandreconstructionforgeneticlev

3、elthatenablebetterapplicationforplantdiseasescontr014368clonesofcDNAlibraryweresequencedbysinglepasssequencingandbioinfomaticanalyzedandtotal3040ESTwereachievedafterdiscardingthesequencesrepresentingvector，rRNA，mitochond

4、rialDNAandshorterthan100bpAfterclusteringanalysis，1281Unigeneswereobtainedwhichconsistof411Contigsand870Singlets486clustersrepresentedknowngenescomparativetoGenbanknonredundatnproteindatabase；theotherclustersshownohomolo

5、gywithnucleotidesequencesdepositedinthepublicdatabasesandthuswereconsideredasnovelgenesChaetomiumUnigeneswerenotedbyrelevantstandardizetermsandBLASTanalysisresultsandsemanticlinkagewasconstructedamongdifferentspeciesdata

6、basebyGOsemanticmodel，onthebasisofwhichcreatingChaetomiumESTbioinformaticsanalysisdatabase，effectivelysolvingintegrationamongvariousspeciesbioinformationonthelevelofconceptandlinkage，andrealizingmultiple，compoundandinter

7、sectantsearchesforChaetomiumbioinformaticsdataintelligentizationAccordingtonewly—builtChaetomiumESTbioinformaticsanalysisdataplatformbasedonGOandintegratedapplicationofvariousbioinformaticsanalysismethods，theauthoridenti

8、fiedachitinaseIIIgeneofclone，131，3一exoglacanasegeneofclone，fucosespecificlectingeneofclone，ergosterinbiosynthesisgeneofclone，cyclicpeptideHC—toxingeneofclonebyanalyzingsyntheticallygenesofbio—controlbymultibioinformatics