一株桃耳七內(nèi)生菌產(chǎn)鬼臼類化合物的研究.pdf

1、y79SB92分類號密級單位代碼10697學(xué)號呈Q魚呈星壘!璺西北大學(xué)碩士學(xué)位論文作者張曉瑞指導(dǎo)教師黃整塹專業(yè)技術(shù)職務(wù)劐螳學(xué)科(專業(yè))絲生塹堂答辯日期星QQ墨!魚學(xué)位授予日期二oo五年六月AbstractPodophyllotoxinisakindofanticancerandinsecticidal1ignansItisaneffectivemedicineintreatingwartandaprecursorofsomeanti—t

2、umormedicineBecauseofitsgreatmedicalpotential，podophyllotoxinhasbeendemandeddesperatelyHowever，thenatural、resourceofitisvery1imitedTherefore，howtoutilizeanddevelopitsresourcereasonablyisahotpointTheexperimenttriestoobtai

3、npodophyllotoxinthroughmicrobialfermentationtosettletheproblemofresourceshortageAnendophyticfungiImlwasis01atedfromSinopodophy]7umcreedl“(Wall)YingThefermentationproductionwasextractedbychloroformandrebutanolandtheextrac

4、tionwascollectedrespectivelyThreecompoundswereseparatedfromtwokindsofextractthroughsilicagelCC(200—300mesh)，TLCandrecrystallizationCompound1wasidentifiedaspodophyllotoxinandcompoundIIaskaempferol，compound111wasinitiallyc

5、onsideredasakindofpodophyllotoxin一0GhtypeonthebasisofTLC，HPLCand‘H，”C—NMRTheexperimentalsostudiedthebiologicalcharacterofthestrainImlTheinitialresultshowsthatImlbelongstoFungiImperfeeti，Moniliales，Demat]aeeaoA1ternariaNo

6、esoxWallImlgrowswellinPDAmediumandhasagoodgeneticstabilityTheoptionalfermentationconditionofImlwasalsoinvestigated：PH60～65，temperature25“CTheresultfullyindicatesthatitisfeasibletouseendophyticmicrobialofanti—caneermedici

7、nalplanttoproduceanticancersubstanceTheresearchpioneeranewgatewayforthedovelopmentofmedicineresource，andithasaveryimportantmeaningtoprotectbiologicalenvironmentKeywords：endophyticmicrobial；fermentation；podophyliotoxin：an