瘤胃纖維素降解微生物的篩選及產(chǎn)纖維素酶酶學(xué)性質(zhì)的研究.pdf

1、南京理工大學(xué)碩士學(xué)位論文瘤胃纖維素降解微生物的篩選及產(chǎn)纖維素酶酶學(xué)性質(zhì)的研究姓名：鄭德申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：生物化工指導(dǎo)教師：楊樹(shù)林20100501Abstract碩士論文AbstractCelluloseisthemostabundantrenewablesourceinthenaturalenvironment，usingmicrobialdeeradationonthefiberresources，andtransforming

2、themintawmaterialswhichcallbeusedforhumanMeanwhilecellulaseisalowcarbonandgreenproteinappliedtopharmaceuticalfieldetcbasedonbiologicalNearlyhalfacenturyCellulasehadbeenresearchedextensivelybydomesticandforeignresearchers

3、，particularlyonthemetabolismregulationofcellulaseproteintheinteractionmechanismsofenzymeproreins，howtoimprovethecellulaseactivityandapplicationofcellulaseetc，andachievedgoodresultsTheseweremainlyfocusonthefungiTrichoderm

4、aastherepresentative，whileresearchoncellulaseproducedbybacteriumwasfewInthispapermicroorganismsofdegradationcellulosewereisolatedfromearlerumenliquid，andabacteriumwithhighcellulaseactivityhadbeenscreenedMainstudyresultsa

5、sfollowing：111widestrainscapableofproducingcellulasewereisolatedfromBlmenliquidToimprovethetraditionalselectivemedium，themodifiedmediumcompositionwasO5％CMC，02％NaN03，O1％K2HP04，005％KCl，005％MgS04‘7H20，00001％FeS04，16％ag鴆whic

6、hcouldreducethephotocopyingprocessThesizeofthehydrolysiscyclecouldbechangedbyadjustingtheCMCconcentrationinmedium2StrainN07wasidentifiedasgenusCellulomonasonthebasisof16SrDNAsequencecomparisonsincombination謝t11morphologi

7、cal，physiologicalandbiochemicalexperiments，namedCellulomonasudaNJ08073ThefermentationconditionsproducingcellulasewereoptimizedbystrainCellulomonasudaNJ0807TheBBD(BoxBehnken)ResponseSurfaceexperimentwasappliedtooptimizefe

8、rmentationconditionsforproducingcellulasebasedonthesinglefactorexperimentUnderthecondition：mediumcompositionof1％peptone，O5％sucrose，1％CMC，O3％NaCl，01％K2HP04，004％MgS047H20，theinitialpH77，5％inoculation23℃，180rman“1for53hours

9、，thecellulaseactivityachieved135UmL～4CellulaseproducedfromthestrainCellulomonasudaNJ0807waspurifiedbyanionexchangechromatographyandgelfiltrationchromatographybasedonFPLCsystem，obtainingthreeendoglucansecomponentsThemolec