抗CD20單克隆抗體與IL-10基因聯(lián)合干預(yù)對(duì)NOD鼠肝臟PDX1及FOXO1的影響.pdf

1、分類號(hào)：R725.8 密級(jí)：不保密UDC： 610 學(xué)校代碼：11065碩士學(xué)位論文抗 CD20 CD20 單克隆抗體與 單克隆抗體與 IL-10 IL-10 基因聯(lián)合干預(yù)對(duì) 基因聯(lián)合干預(yù)對(duì) NOD NOD 鼠肝臟 鼠肝臟 PDX1 PDX1 及FOXO1 FOXO1 的影響 的影響范 磊指 導(dǎo) 教 師 李堂教授學(xué)科專業(yè)名稱 兒 科 學(xué)論文答辯日期 2016 年 5 月 20 日Effect of Combined Interventi

2、on with Anti-CD20 Antibody andInterleukin-10 Gene on Liver PDX1 and FOXO1 Expression in NODmiceAbstractObjective:to observe the influence of liver PDX1 and FOXO1 expression in nonobese diabetic mice after interfered by a

3、nti-CD20 monoclonal antibody combined with inter- leukin10.Methods:Twenty-four female NOD mice were randomly divided into four groups. MouseA:Anti-CD20, B:Anti-CD20+IL-10,.C:IL-10 and D:Normal Control(NC) groups were cau

4、dal venously injected of 250ug Anti-CD20 antibody, combined Anti-CD20antibody 250ug and 0.1ml IL-10，0.1ml IL-10 and 0.1ml physiological saline，respectively. But the first day,A and B groups were injected Anti-CD20 antibo

5、dy 500ug. All mice were monitored for blood glycose every day , and sacrified 6weeks after injection. Their serum levels of C-Peptide,TNF-α,IL-10,CD20 were measured by ELISA.The liver PDX1 and FOXO1 expressions were obse

6、rved by immunohistochemistry、western blot and RT-PCR.Results: After combined Anti-CD20 and IL-10 intervention, the C-Peptide in the serumwere enhanced(P＜0.05) and the serum TNF-α were lower compared with other 3groups(P＜

7、0.05). The liver PDX1 expression was upregulated(P＜0.05) and the theexpression of FOXO1 in the liver was decreased (P＜0.05).Conclusion：The combined administration of Anti-CD20 and IL-10 increased theexpression of PDX1, a