溫郁金藥渣化學成分研究及栽培白術種質遺傳多樣性分析.pdf

1、溫州醫(yī)學院碩士學位論文溫郁金藥渣化學成分研究及栽培白術種質遺傳多樣性分析姓名：馬喜彥申請學位級別：碩士專業(yè)：中藥學指導教師：董建勇20100501溫州醫(yī)學院碩士學位論文StudiesontheConstistuentsofDregsfromCurcumawenyujinandAnalysisofGeneticDiversitiesofCulturedAtractylodesmacrocephalaABSTRACT‘≮Thethesisi

2、ncludestwoparts：thefirstpartiSaboutstudiesontheconstistuentsofdregsfromCurcumawenyujinYHChenetCLing；thesecondpartisaboutanalysisofgeneticdiversitiesofculturedAtratylodesmacrocephalaInthisthesis，thechemicalconstituentsoft

3、hedregsfromCurcumawenyujinYHChenetCLingwerestudiedOnthebasisofdifferentkindsofmordenchromatographyandidentificationmethods，8compoundsweredetermined，theyweresesquipenestheirstructureswereidentifiedascurcolide(1)，curdione(

4、2)，aerugidiol(3)，neocurdione(4)，alismoxide(5)，procurcumenol(6)，zedoalactoneI(7)，gajutsulactoneD(8)Inconclusion，thisreasarchhasprovidedrationalandexperimentialbasisforthecomplexutilizationofthisdregsofCurcumawenyujinYHChe

5、netCLingAtractylodesmacrocephalaisamemberofAtractylodesmacroephalaKoidz，whichisdistributioninZhejiangProvinceThecultivatedAtractylodesmacrocephalawerecollectedfromsixareasofTiantai，Xinchang，Panan，Dongyang，Shengzhou，Xianj

6、uinZhejiangProvincebytheprinciplesofuniformdistributionandrandomsampling6individualswerecollectedforeachtwonand36individualsintotalThecollectionregionsshouldCOVertheproductionareasaswidlyaspossibleAFLPwerecarriedouttoana

7、lyzethegeneticdiversityofcultivatedAtractylodesmacrocephalainthesesixpopulationsThepocessofAFLPisasfollows：RestrictionoftheDNAandligationofoligonucieotidcadapters；Selectiveamplificationofsetsofrestrictionfragments；Gelana

8、lysisoftheamplifiedfragments12606bandsweregeneratedby7pairsofAFLPprimercombinations，ofwhich10044werepolymorphic，thepercentageofpolymorphicWas7969％ThevarietyrangeofPPB％amongdifferentpopulationsis5417％5926％，5671％inaverage，

9、iSbetween02426“02646，02600inaverageNeiSbetween12549““12782，01648inavrageGstis00970，Nmis46547Thereisarelativehi曲geneticdiversitieslevelinZhejiangProvinceculturedAtractylodesmacrocephalaGeneticdifferentiationexistsamongpop