貴州省部分主要甘藍(lán)型油菜雜交種及其親本指紋圖譜構(gòu)建與純度鑒定.pdf

1、貴州大學(xué)碩士學(xué)位論文貴州省部分主要甘藍(lán)型油菜雜交種及其親本指紋圖譜構(gòu)建與純度鑒定姓名：馬曉峰申請學(xué)位級別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：王通強(qiáng)20060501基捌太堂亟主堂焦迨塞蚩趔笪整僉主要苴墮型迪墓盤鑾弛厘甚塞奎i旨筮圖譜掏建皇綞廑鑒定AbstractInthisstudy，morphologymethodandtheSSRmarkermethodwereusedtoanalyze43brassicanapusmaterialsi

2、ncludingguizaserieshybridandtheirparentsetcDifferencesbetweenthetwomethodwerediscussedHighmulti—statepropertySSRprimerwereselectedandusedinbuildingDNAfingerprintingdatabaseofguizaserieshy梳dandtheirparentsThemainresultswe

3、reasfollows：1Morphologymethodwereusuallyusedtoobservecharacteristicofbrassicanapushybridsandtheirparentsinseedidentificationandpuritybutthismethodisnotexact224bmssicanapusinbredlineswereanalyzedbyusingSSRtechnicsTwenty—o

4、nepairsoutofonehundredpairsofSSRprimerswereselectedtogavepolymorphic，stableandrepeatedamplificationprofilesFourprimersincludingNal2C06、Nal0804b、0110A05、Nal0D03wereselectedtobuildtheDNAfingerprintingdatabaseofall24inbredl

5、ines3DNAfingerprintmapof19brassicanapushybridswerebuiltwith21pairsSSRprimersTheseprimersalsogavepolymorphic，stableandrepeatedamplificationprofilesFourprimersincludingNal2C06、Nal0804b、0110A05、Nal0D03wereselectedtobuildthe

6、DNAfingerprintingdatabaseofall19brassicanapushybrids4TestinthisresearchprovedthatSSRtechnicsCanbeusedinidentifyingthepurityandrealityofbrassicanapushybridsquicklyandprecisely5UseingprimeringroupmethodCanexpandthecoreprim