太原地區(qū)五歲以下腹瀉住院兒童輪狀病毒的分子流行病學(xué)研究.pdf

1、山西醫(yī)科大學(xué)碩士學(xué)位論文太原地區(qū)五歲以下腹瀉住院兒童輪狀病毒的分子流行病學(xué)研究姓名：蘭蓓申請學(xué)位級(jí)別：碩士專業(yè)：流行病與衛(wèi)生統(tǒng)計(jì)學(xué)指導(dǎo)教師：李佩珍20100505pq醫(yī)科人學(xué)碩I學(xué)何論文MolecularEpidemiologyofRotaVirusamongchildrenunder5yearsoldhospitalizedfordiarrheainTaiyuanAbstractobjectiVes：Tbstudytheinfecte

2、dinformation，clinicalsymptomarldnnmolecularepidemiologicalcharacteristicsofHRVinfection鋤onghospitaliZedchildrenwithacutedia玎heaunder5yearsoldin1’aiyuan，fbm2007to2008；tocoreectthebaCkgrounddatarelatedwithviraldi礎(chǔ)ea；topmVi

3、dereferencesforpreventionandtreatofthediseaseMethods：Atotalof346stools、verecollectedfromhospitalizedchildrenwithacutediarrhea明der5yearsoldinTaiyuanbet、^，een2007，llto2008，l0，RotaVimspositiVespecimensⅥ，ereidentifiedbyELISA

4、kitG／PtypingaSsayswereconfirmedwithmultiplexseminestedfmPCRThesepositiVeproductswerepurified，extractedplasmid，thenselectedthespeciemeswhichwereconfinnedbyplasmidPCRtesttobesequencedthenucleicacididentifythegenotypeandgen

5、ogroupofaVailablestrainsResulits：Atotalof346stoolswerecollectedfbomhospitalizedchildrenwithacutediarrheaunder5yearsoldinTaiyuanbet、veen2007，1lto2008，lOOfthe346specimens，thepercemageofsamples謝thI沁taVimswaus408％Among14lrot

6、aVimspositiVesamples，sero哆peGl(426％)wasthepredominaIlts仃ainfollo、ⅣedbyG3(262％)，G9(106％)andmixedGinfection(64％)142％ofstrainsremainedt0benontypeablePgeno帥ingshowedP[8】(681％)waSmostcommonfollowedbyP【4】(07％)，andnon—typeable(

7、241％)ThemostcommoncombinationofGandP、VaSGlP【8】(319％)，foIIov，edbyG3P[8】(184％)a11dG9P[8】(71％)M0rethan957％ofViraldiarrheapatientsunderhospitalizationoccurredamongchildrenyoungerthan2yearsConclusions：RotaVimsremainstllemosts

8、ignificantViralagentcausingdiaHheahospitalizationamongchildrenundermeageof5yearsinTaiyuanThemostcommonchildrenwithrotavimsinfectionswereyoungerthaIl2yearsoldRVpreValencepeakedfromSeptembertoNovember7Ihepredominantstminso

9、frotaViruswereG1andP[8】inTaiyuancityInformationonthediVersityandcomplexityofRVstrainscirculatinginTaiyuanproVidesusefuldataforfo硼ulatingVaccinepolicyandeValuatingVaccinee伍cacyKeywords：rotaVims；diallrhea；molecularepidemio