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1、陜西中醫(yī)學(xué)院碩士學(xué)位論文中藥平消栓對(duì)H肝癌移植瘤模型小鼠bcl-2和TNF-α表達(dá)影響的實(shí)驗(yàn)研究姓名:王裕云申請(qǐng)學(xué)位級(jí)別:碩士專業(yè):中西醫(yī)結(jié)合臨床指導(dǎo)教師:孔平孝20100401The Experimental Research of Ping Xiao Suppository Effected The Expression of Bcl-2 and TNF-αin ICR mice Transferred by H22 Abstrac

2、t Objective Objective Through the research of Ping Xiao Suppository to H22 ascites carcinoma tumor lines transfected ICR mice the general projects, immune organs(thymus,spleen),bcl-2 and TNF- α expression of experimenta

3、l studies to explore the possibility of anti-tumor drug mechanism and for the further development of Chinese Medicine in cancer research and provide a theoretical basis. Method Method Based on the randomi

4、zation principle, 40 ICR female mouse divides stochastically into four groups, respectively be the blank control group(group A),the Dutch lump control group(group B), 5-fluorouracil group(group C),the Ping Xiao

5、 Suppository group(group D).Each mouse in The group B, group C and group D has vaccinates the H22 lump cell at the right armpit by the hypodermic to hang fluid 0.2ml.After 24 hours, weigh each mice and start to abd

6、ominal cavity injection to give the medicine. Group A and B use physiological saline 0.2ml/ each /every day(continuously to give medicine 14 days). Group C use 5-fluorouracil 25mg/kg, dilutes the density to mg/

7、0.2ml (give medicine D1-D5), Group D use Ping Xiao Suppository which dissolved in the physiological saline, 1.0g/kg (continuously to give medicine 14 days). Daily observation of mice eating, drinking, activitie

8、s, mental state, such as hair color and death, a detailed record of changes in average body weight in mice drawing curves. Dislocation execution the mouse at the 15th day, take the tumor tissue, thymus, spleen

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