整合素α-,5-、β-,3-在子宮內(nèi)膜異位癥中表達的研究.pdf

1、東南大學碩士學位論文整合素α、β在子宮內(nèi)膜異位癥中表達的研究姓名：成杰申請學位級別：碩士專業(yè)：婦產(chǎn)科學指導教師：任慕蘭20050510末南大學項士學位論文EngIishAbstractsInvestigationofintegfino5andinteg“nB3inendometriosisCHENGJleProfessorRENMula”Cllnjca【S。h。叫。fMedjca】Science，Southeast乙nlver5lty0

2、bjectives：TosLudylhee坤ressionands把roldalregul曲onofmtegrln“5ar|dIntegnn魁ineutoPlccndometrIumandectopicendometum；andtolnvestl耐ethec何證ctsofestradol、口mgesterone、anties廿adjo】andant】progesteroneonthcexprcssionofjnte汀in嘶、B1mRNA

3、jnculturedhumanendome仃joticcellsinvj仃oA11o九hesepurposesa心t0seachforthee抒色cesofjntegrlnonendometnosislsoccurrenccanddeveloDme卅，a：nddoourefronsforcndomcⅢosls’sdia印oslsandⅡe趾丌1entMethods：lCulturedhlmlanendometrioticcellsinv

4、j橢：(nEctoDicendome仃ialtlssuesweredigestedbyenzvmolo舀calteohnjcsandcu】tured1nvltroThecultu咒dce】】sweredentl行edbymouse—antl一humankerat，nantmodyandanayzcditspumvf2)Thee毹ct5ofes”adioIrE，)、prozesterone(P)、tamo州fcn(TAM)andm1把pr

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6、ndnion(4)Es訂adio】(E2，10nmoUL)、proEes舊0力e化100nmol幾)、ta】110xjfenrTAM，1000nmol幾)andmlfepnston(Ru486、】000nmo】，L)anedonthecuIturedectopicendome仃la】cel】sfespectjvelvfor57davs1、heexp螂sjonofin捃g—na艮mR姐ofeachgr。up計℃mde把rminedbvre

7、坩rse打aflscn酣pojymerasech酣nreactlon(RT_PCR)Ⅱ1etIod2ClInicpaIt：(1)RTPcRmethodwasutj】1zedtodetectandquanttatcexpression【eveIsofIntegrino”B3mRNAl力eutoplc卸dcctopcendometrⅫtjs5ue5from18D砒ie”tswthendoⅡ螗一osis(stlldyg|oup)蜘d18non

8、endometnoslswomon(con打olgroup)(2)serumconcentr鰣onsofestradlol(E2)、progeSterone(P)andcAl25wcremea5uredbyradiolmmunoassayEveryAFS—rofthestateofpelvicc馴itywasscoreddullngoperanonResuI扛(1)TheraceofsuccessfulculⅡlringectopice

9、ndome打ialcellsinvl廿oacllieve444％(8／18)(2)Es廿adlol(10m01幾)andpr。ge㈣one(100nrnol，L)showeds酬矗canflye恐ctsonsclm咖噸theBrowthofectoplcendome廿ialcellswhereas，tamox】fcn(1000nnl01／L、andmlfep“ston(1000nmoj幾)showedsiEni矗carIUye飪cts0

10、111nhiblnngthegrow伽ofectopicendome竹ialcellsThcrewasacenainrelatjonshi口betweendru窟’sdensitvand盯Iivjlvofendometria】cellsf3)TheexPresslonofintc烏rin05mRNAinE28ndP盯uupgot1415上0286and1_3040170respectivelY，which、牝reS】gni打cantly

11、hi曲erthanthaIofcon竹olgroup(1082O069)，(PO05)lnTAMandRU486group，itwas0882O叭3卸dO8660054respcctiveiywhichweres瞧川竹cantjyJower脅an山acofcoll廿oJgroup曠005)T№ex口reSsionofintegrinB3mRNAinE2andPgfoupwas0J844O003and08030005，whichweres

12、igni6cantjyhigher小aIltllatofcolltrol母oup(0703O009)，(，005)InTAMandRu486酊ouP，itwas06040003andO5920們lrespectively，whichwcrc副仃n訊cantlylowerthanthatofcon訂o】group妒005)(41Thevariou5Posjtjved。greesofexpresslonofin記grinsubunits毗a

13、nd口3weresh。wJlinbotl]eutoplcandec‘opicendome研a1tlssuesofendometrtosjsandnon、endomc州oskTheexpressionsofime母jn砒mRNAmeu船piccndometrum，ec幻pjcendome盯2um肋d朋dometrjumofcontr。Jgfoupw甜e0773L01I3，10770m2and09240120respeo【ivdy，吐ler

14、ewass1靜1I“canldlmrencebetweenevery竹。阿吼】ps(PO05)Moreoverl1eexpresslonofIntegrinB1mRNAineutopjcendometrium，ectopiccndome”iumande11donleⅢumofcontrolgroupweres0653O080，0963O】32and07630090respectivelv，therewassigni稈cantdifrer

15、encebdwccncverytwogroups(Pt：005)(51SerumconcenⅡaeionofE2insIudv2loupwasposijvecorrelatedwiththeex口ressionlnlezrinⅡ50=o793，尸005)andB巾=O793、O，917，尸O05)Ⅵ7hi】cserumconce爪m!fonofPhasno雎】evancecojflfogrmaiandPBothfeveJof50刪mCA

16、，iandscoreofAFS—rhwepu血ivecorfelat】onw‘Ihmtegrin啦andB3(，O05)conclusion：IntegrIndEand口1havesomeeffectsonendomotriosis’soccurrenceanddevelopment(1)FstradiolandprOgestemnecanpromotetheErowll】ofeclopicendometrlalce】ls，whlJeo