多色熒光稀土納米顆粒的制備與應(yīng)用.pdf

1、大連理工大學(xué)碩士學(xué)位論文多色熒光稀土納米顆粒的制備與應(yīng)用姓名：蔣鴻飛申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：應(yīng)用化學(xué)指導(dǎo)教師：袁景利20090601多色熒光納米稀土顆樁的制備與應(yīng)用PreparationandapplicationofmulticolorluminescentlanthanidenanoparticlesAbstractTimeresolvedfluorescence(TRF)bioassaytechniquebasedonlongli

2、vedfluorescenceoflanthanideprobesisanultrasenstivebioanalyticalmethod，andhasbeenwidelyusedinclinicaldiagnosticsandbiotechnologyBecausestronglyfluorescentlanthanidecompoundsalemainlylimitedtoEus十a(chǎn)ndTb”compoundswithred(Eu，

3、“15nm)andgreen(Tb，～545nm)emissions，thedevelopmentandapplicationoffluorescencebioassaytechniqueusinglanthanidebasedmulticolorfluorescentbiolabelsarerarelyinvestigatedInthisworkaseriesoflanthanidecomplexesboundsilicananopa

4、rticleswithred，orange，yellowandgreenfluorescencecolorswaspreparedbycobindingdifferentratiosofEu3十廠rb“fluorescentcomplexeswiththeligand(4’phenyl2，2’：6’，2“terpyridine6，6“diyl)bis(methylenenitrilo)tetrakis(aceticacid)inside

5、thesilicananoparticlesCharacterizationsbytransmissionelectronmicroscopyandfluorometricmethodsindicatethatthenanoparticlesaresphericalanduniforminsizewimlongfluorescencelifetimes(10ms)andhigllphotostabilitiesThenanopartic

6、le—labeledstreptavidinWaspreparedandusedfortimeresolvedfluoroimmunoassayofhumanprostate—specificantigen(PSA)Themethodgivesadetectionlimitof021ng／mLforhumanPSAshowingthatthenanoparticlelabeledstreptavidinstillhasagoodbio—

7、reactivityThenanoparticle—labeledstreptavidinWasfurtherusedfortimeresolvedfluorescenceimagingdetectionofanenvironmentalpathogen，Giardialamblia，inthewatersamplesTheresultsindicatethatthenewnanoparticlesareusefulmulticolor