具有壓縮DNA能力的雙溫敏性聚甜菜堿型ABA嵌段共聚物.pdf

1、天津大學碩士學位論文具有壓縮DNA能力的雙溫敏性聚甜菜堿型ABA嵌段共聚物姓名：代鳳英申請學位級別：碩士專業(yè)：材料學指導教師：劉文廣20080601ABSTRACTABA帥eM口DsAHyME02MA。MPDsAHyw油narrowpolydispersi哆i11dex、VaspreparedbyATRP訪themi)(tureofwater／methanolwithaddjtionofsodiumchloride’nleaqueous

2、so】utionofcopo】ymerwasshowntoe)dlibitUCSTandLCSTbehaviorsThedualtemperaturesensitiVenesswaSi11VestigatedViaturbidi夠measurement，Variabletemperature1HNⅣ陬aswellassteady—statefluoresenceTheUCSTWasd印endentuponthesolutionconce

3、n的tionandtIleblocklengthofMPDSAHMicropolari夠aSsayofpyrenerevealedthatintheselectedlowtemperatureregion，tIlemicropolarityofpyreneslightlymcreasedduet0weakiIltemctionofpositiVe—negatiVeinteractionindilutedsolution；whileabo

4、veLCST，pyreneexperiencedmorehydrophobicmilieuowingtothenoticeabledehydrationofPME02MATheanalsysisofethyidiumbromidedisplacementsuggetedthecapdb訂i砂ofⅣ口DSAHhomopolymerandcopolymerStobindDNA99％EBcouldbereplacedbyMPDSAHhomop

5、olymerME02MAmoietiesincopolymerSweakenedthebindingabilityofPNmDSAHtoDNA，but5460％EBwasr印lacedbycopolymeratacriticalcomplexingmtioAFMconfinnedthatPⅣ中DSAHandcopolymerswerecapableofcondensingDNAtonanaoparticlesatanappropriat

6、ecomplexillgratiosThetemperaturesensitiVenessofpolymer／DNAcomplexwas抽VestigatedviacloudmeasurementComplexingwithDNA，UCSTofsolutionvanished，butLCSTwassli曲tlyiIlcreasedduetotlleeIlhancedhydrophilicitycausedbyliberationofne