單分子計數(shù)定量方法研究.pdf

1、山東大學(xué)碩士學(xué)位論文單分子計數(shù)定量方法研究姓名：許光申請學(xué)位級別：碩士專業(yè)：分析化學(xué)指導(dǎo)教師：姜瑋20050515山東大學(xué)碩士學(xué)位論文AbstractThisthesisincludestkreeparts：DetectionofsinglebiomoleculesDetectionofsingleantibodymoleculesinsolutionbytotalinternalreflectionflunresccncemicros

2、copyDeterminationofgoatandratIgG(H十L)bycountingsinglemoleculesInthechapteroneofthisdissertation，thedetailreviewofdetectionofsinglebiomoleculeswasdeliveredSignificance，principleandbasiccontentofsinglemoleculesdetectionand

3、strucRne，principleofmicroscopeinsinglemoleculesdetectionwereintroducedActualitymethodofsinglemoleculesdetectionw眥introducedinaspectoftracingsinglemolecules，singlemoleculesenzymologydynamicsofmolecularconformationmotionof

4、motorproteinionchannels，cellsignalconductiondetectionofspecificDNAsequencesatthesin#emoleculelevel，countingofsinglemoleculesInthechapte‘81referenceshavebeenreferredInthechaptertwO，themethodofdetectionsinglegoatantiratIgO

5、(HL)moleculesbytotalinterm／reflectionfluorescencemicroscopyWasinvestigatedSeveralparametersincludinglaserpowerexposuretimewasoptimizedforimagingofsinglegoatantiratIgG(HL)moleculesThefollowingconditionsw般suitable幻ftheimag

6、ingofsinglemolecules：laserpower24kW；exposuretime100msReducebackgroundfluorescencebypre—photoblunchingbufferafterfiltrationprephotoblanchingcoverslip，reducingexcitedvolume，makinguseoffittingcubes，increasedetectionsensitiv

7、itythenimagingsinglegoatantiratIgGmL)moleculesbyICCDTherearetwoevidencesforprovingsinglemoleculesThemolecularnumberislinerwithgoatantiratIgO(HL)concentrationintherangeofIOX10。10moYL4O10“qmol／LTIlisisnotonlyapotemevidence

8、forsinglemoleculesbutalsoamethodfordeterminationofproteinhlthechapterthreeAnovelmethodfordeterminationofproteinwaspresentedcoverlipsWerOdealedwithglycidoxypropyltrimethoxysilaneandepoxygroupWascreatedORthesurfaceofcovcrl