一株產(chǎn)卡拉膠酶南極菌alteromonassp.r115的篩選、優(yōu)化培養(yǎng)及酶學(xué)性質(zhì)的研究

1、 分類號： 分類號：Q93 Q93-33 33 密級： 密級：不保密 不保密 UDC UDC：579.2 579.2

2、 學(xué)校代碼： 學(xué)校代碼：11065 11065 一株產(chǎn)卡拉膠酶南極菌 一株產(chǎn)卡拉膠酶南極菌 Alteromonas sp. R11-5 的篩選、優(yōu)化 的篩選、優(yōu)化培養(yǎng)及酶學(xué)性質(zhì)的研究 培養(yǎng)及酶學(xué)性質(zhì)的研究 王蕾 王蕾 指 導(dǎo) 教 師 張培玉 教授 學(xué)科專業(yè)名稱 生態(tài)學(xué) 論文答辯日期 2017.06.05 Abstract In this paper, 12 strains with carrageenan acti

3、vity were screened from 85 strains of Antarctic bacteria, and the highly active strain R11-5 was identified and optimized for its fermentation. The whole genome was sequenced and sequenced. Carla gelatin gene Car1853, an

4、d its efficient expression; purification of recombinant carrageenan enzyme, the system of its enzymatic properties, analysis of the degradation characteristics of the enzyme, to clarify its degradation products and thei

5、r type, with a period of carrageenan and carrageenan Sugar industrial production to provide theoretical and technical support. In this experiment, the newly identified high activity carrageenan strain R11-5 was identifie

6、d, and the results of morphological and 16S rDNA species showed that the Antarctic bacteria belonged to Alteromonas. The optimum culture conditions were as follows: temperature 15.7℃, pH7.0, yeast extract 0.6%, beef extr

7、act 1.17%, carrageenan 1.05 ‰, CaCl2 5.78 mmol/L, The maximum enzyme activity was 56.972U ·mL-1, and the activity of enzyme was 1.7 times higher than that of the control group. The recombinant plasmid pET30a-Car1853

8、 was constructed by gene engineering, and the recombinant plasmid pET30a-Car1853 was transformed into Escherichia coli BL-21. The recombinant plasmid pET30a-Car1853 was constructed by gene engineering. SDS-PAGE showed th

9、at the molecular weight was 42 kDa. The results showed that the optimum reaction temperature was 55 ℃, the optimum pH was 7.0.The ion has an inhibitory effect on its activity. Thin layer chromatography showed that the fi