陸地棉雙列雜交的遺傳效應(yīng)及表達譜分析.pdf

1、中國農(nóng)業(yè)科學院博士學位論文陸地棉雙列雜交的遺傳效應(yīng)及表達譜分析姓名：楊代剛申請學位級別：博士專業(yè)：作物遺傳育種指導教師：喻樹迅20110520AbstractCottonisthemostimportantfibercropinChinaandthewodd，anduplandcottonaccountsformorethan95％cottonproductionYangtzefiverbasinYellowriverbasinandX

2、injianga他thethreemajorcottongrowingregionsinChinaStudyofthegeneticeffectsandtheexpressionprofdeofuplandcottoncultivarsfromthethreemaingrowingareasshouldbehelpfulforselectionforthebackboneparents，heterosispredictionandmol

3、ecularbreedingincottonInthisstudy，10representativeuplandcottoncultivarsfromChina‘SthreemajorcoRon—growingregions，usingadiallelmatingdesignmethod，wereusedtoproduce45F1hybridsandtheir45F2generationsHeldexperimentswerecarri

4、edoutin2008，2009and2010，inthreetypicalecologicalregionsforcottonproductioninChinaincludingWangjianginAnhuiProvince，AnyanginHenanProvinceandAksuinXinjiangUyghurAutonomousRegionEightmorphologicalandyieldtraitswer；cinvestig

5、atedandfivefiberqualit)，characters嘴surveyedGenemicroarrayanalysiswasconductedusingfloweringbudsatfullbloomstagein2008StatisticalanalysisWasconductedusingthegeneticmodelincludingadditivedominanceepitasisanditsinteractione

6、ffectwithenvironm蚰tconditionanalysismethodQTLNetworkV30Themainresultsaleasfollows：(1)Fortheeightmorphologicalandyieldtraits，themaingeneticeffectsandtheirinteractionwithenvironmentplayaroleatvaryingdegreesAdditiveby—addit

7、iveepistaticeffectsonbollnumberperplantbollweightlintpercentage，seedindexandlintyield，interactionbetweenadditivoadditiveepistaticeffectandenvironmentonlintpercentageandseedindexwerepositivelysignificantGeneralheritabilit

8、yinthenalTOWsense(l芻)forbollweightlintpercentageandseedindexwerehigh，indicatingthatearlygenerationselectionWaSeffectivePlantheight，numberoffruitingbranchesandbollnumberperplantweregreatlyinfluencedbyenvironmentindicating

9、thatdirectselectionislessreliableInteractionheritabilityinthebroadsense(日品)for舭numberoffruitingbranches，bollnumber。seedcottonyieldandlintyieldwererelativelylarge(314％一518％)，indicatingthatheterosisCallbeusedinspecificenvi

10、ronmentsParents9018，CCRl41，sGK958and2028callbeusedashybddparentstoconcurrentlyimprovetwoorthreeyieldcomponents(especiallylintpercentage)，leadingtosignificantlyincreaselintyieldTheadditiveeffectandadditiveaddtiveepistasis

11、Offlintpercentageandseedindexinparent2028Waslargeandpositivelysignificant，andshouldbeusedinselectionbreedingUsing339215455asparentwasexpectedtoeffectivelyimprovebollweightandthusincreaselintyield(2)Forthefiberqualitytrai

12、ts，boththemaingeneticeffectsandtheirinteractionwiththeenvironmentplayarolebutthedifferencew蠲largerFiberstrengthwagmainlyaffectedbyadditiveeffects，whichcallbegeneticallyimprovedbyconventionalbreedinghGZandgeneralheritabil

13、ityinthebroadsense(n。2；)ofupperhalfmeanlengthwe∞large，andearlygenerationselectioncanalsobeconductedandheterosiscanbeutilizedinspecificenvironmentve|vPofuniformityindex。elongationandmicronairevaluewereabove40％，whichnleans