27242.擬南芥組成型抗病突變體snc1對(duì)二氧化硫脅迫的應(yīng)答

1、沈陽師范大學(xué)碩士學(xué)位論文擬南芥組成型抗病突變體snc1對(duì)二氧化硫脅迫的應(yīng)答姓名：許佳申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：郝林20100301iiArabidopsisMutantsnc1acterizedwithConstitutiveResistancetoPathogensResponsestoSO2ExposureAbstractSulfurdioxide(SO2)akindofmainairpollutants

2、recognizedasharmfultohumanbeingsecosystems.Practiceshowsthatplantisanidealtooltomonitairpollutionclearoutthepollutant.InthisexperimentArabidopsiswildtype(Columbia)itssalicylicacid(SA)alteringmutantssnc1(suppressofnpr11co

3、nstitutive)withhighSAleveltransgeniclinenahGwithlowSAlevelnpr11(nonexpressofPRgene)withSAsignalingblockagedoublemutantsnc1nahGplantswereusedtorevealthemechanismbywhichthesnc1mutantresponsestoSO2exposureincontrolledcondit

4、ions.AllthetestedplantswereromlydividedintotwogroupsreferredascontrolgroupSO2exposedgroup.FSO2exposureplantswerefumigatedwith1.429mgm3SO2inaexposedchamberf8h(8:00–16:00)perdayf7days.TheconcentrationofSO2usedwasderivedfro

5、mrelatedliteratures.ChamberSO2concentrationwasmonitedduringtreatmentbymeasurementbypararosanilinehydrochlidespectrophotometry.Fthecontrolgroupfilteredairalonewassupplied.AttheendoftheSO2exposuredatawerecollectedonrelativ

6、ebiomassincreasephotosyntheticrateintercellularCO2concentrationstomatalconductanceenzymeactivitiesofsuperoxidedismutase(SOD)peroxidase(POD)catalase(CAT)inleavesmalondialdehyde(MDA)contentelectrolyteleakage.UnderSO2unstre

7、ssedconditions(coalfilteredair)relativebiomassincreaseinsnc1was58%whilethevaluevariedbetween76%82%inotherlinesindicatingthatthehighSAlevelinsnc1inhibitedplantgrowth.HoweverunderSO2exposurethevalueinrelativebiomassincreas

8、einsnc1plantsdecreasedonlyslightlycomparedtotheunstressedcontrolwhileadramaticreductionoccurredinotherlines.ExposuretoSO2inhibitedphotosyntheticrateofalltestedlineswithnahGnpr11snc1nahGplantsbeingmostseverewhilesnc1plant

9、sshowntheleastdifferencefromtheSO2unstressedcontrol.IntercellularCO2concentration(Ci)wasrelevanttothephotosyntheticrateinallthelines.SO2exposuresignificantlyreducedthestomatalconductanceofalllinesexceptthesnc1whereitwasa

10、ffectedonlyslightly.ExposuretoSO2suppressedSODactivityinalllinestestedwithnahGnpr11plantsbeingmostseverewhilesnc1plantsdecreasedslightly.PODactivitywasincreasedbySO2exposureinalllineswhilesnc1plantsaffectedsignificantly.