26420.磁性熒光納米粒子分離家蠅gaba受體蛋白

1、上海師范大學(xué)碩士學(xué)位論文磁性熒光納米粒子分離家蠅GABA受體蛋白姓名：周健申請學(xué)位級別：碩士專業(yè)：有機化學(xué)指導(dǎo)教師：任天瑞李興玉20100401上海師范大學(xué)碩士學(xué)位論文AbstractInsectGABAreceptorisatargetforpesticidesanditisinstructivetostudythestructureofinsertGABAreceptorproteinstodevelopefficientlowto

2、xicitypesticidesInthispaperaccordingtothelatestresearchinmaterialscience，magneticfluorescellano—particleswerepreparedtoseparatehouseflyneuroncellsandorganismGABAreceptorproteinThesharpofhouseflyneuroncells、theweightofhou

3、seflyandmouseGABAreceptorproteinandtheirbiologicalcharacteristicswereresearchedMagneticfluorescenano—particleswerepreparedbyreversemicroemulsionandligandGABAwasmodifiedonthemagneticfluorescenano—particlessurfacePhysicala

4、ndchemicalpropertiesofmagneticfluorescenanoparticleswerecharacterizedbyinfraredspectroscopyatomicforcemicroscopytransmissionelectronmicroscopyvibratingsamplemagnetometerandBETsurfaceareaanalyzerLeukemiaK562cellsastargetc

5、ells，thebiologicalsafetyofmagneticfluorescenanoparticlesweredetected，theresultofwhichshowedthatmagneticfluorescenano—particleswerenotoxicityonK562cellsTheligandofmagneticfluorescenano—particleswereusedtospecificcoupled、Ⅳ

6、itllhouseflyGABAreceptorwhichwasonthesurfaceofhouseflyneuroncells，houseflyneuroncellswhichWaslabeledbymagneticfluorescenanoparticlecomplexeswereseparatedunderthemagneticfield，theaxonstructureofneuroncellsCanbeobservedcle

7、arlyunderthefluorescencemicroscope，whichWasidentifiedasneuroncellsbycollatedtheliteratureGABAreceptorproteinsweregainedafterisolatedneuroncellsandpurificationandthemolecularweightofwhichWasdeterminedbySDS—PAGEHouseflyGAB

8、Areceptorprotein，comparedwithmouse，theresultsofSDS—PAGEshowedthatthehouseflyandmouseGABAreceptorbothhavethreekindsofdifferentsubunitsTheinnovationofthispaperisusingmagneticfluorescenano—particlecomplextoseparateneuroncel