21356.黃山風景區(qū)常綠闊葉樹生齒裂菌屬分類學(xué)研究及該屬種間遺傳多樣性分析

1、安徽農(nóng)業(yè)大學(xué)碩士學(xué)位論文黃山風景區(qū)常綠闊葉樹生齒裂菌屬分類學(xué)研究及該屬種間遺傳多樣性分析姓名：賈國軍申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：林英任201106iiAbstractHuangshanscenicspotlocatedinsouthernAnhuiProvienceisthemainlscapeareaofourcountry.Thecomplexterrainmildtemperatureplentifulrainfal

2、lmakeitsuitablefreproductionofnumerousplantsCoccomycesspeciesarewidelydistributedaswell.MembersofCoccomyceswhichcollectedfromtheevergreenbroadleavedtreesofHuangshanscenicspotweresystematicallystudiedwiththemostadvancedpo

3、lyacterintegratedtaxonomymethodfocusedontheactersofmphologydevelopmentalbiologyecologydistributionetc.Meoverthemethodofthenucleotidesequencesofinternaltranscribedspacer(ITS)wereusedtoinferphylogeicrelationshipsofthe18Coc

4、comycesstrains.Resultsdeterminedtheimptanceofsomemphologicalactersthecategydiversityofhostsontheclassificationoffungalspecieslevel.RelationshipsgeicdiversityoftheinterspecificstrainsofCoccomyceswerefurtherconfirmed.Speci

5、mensofCoccomycesontheevergreenbroadleavedtreescollectedfromHuangshanscenicspotownedbytheHerbariumofFestFungiofAnhuiAgriculturalUniversity(AAUF)wereclassifiedidentifiedaccdingtothetaxonoicprinciplesofSherwood(1980)Johnsto

6、n(1986)Spooner(1990)Kirketal.(2008).13specieswereindentifiedincludingCoccomycescyclobalanopsisC.deltaC.huangshanensisC.illiciicolaC.leptideusC.limitatusC.magnusC.minimusC.mucronatusC.multangularisC.occultusC.sinensisC.sy

7、mploci.AmongthemC.minimuswasanewspeciestobepublishedlatindiagnosesweregivenaswell.Mphologicalacteristicshabitshostsdiscussionswereprovidedfeachspecies.Dichotomouskeytothesespecieswerecomplied.Inthisthesisfreshmaterialsof

8、CoccomycescollectedfromHuangshanscenicspotTiantangzhaiNationalFestParkWanfoshanNationalFestParkwereisolatedcultivatedbytissueisolationtechnique13purestrainswereobtained.DNAofallthestrainswereextracteddetectedrDNAITSregio

9、nswereamplifiedbyPCRthensequenced.GCcontentof18strainswereanalysedbythemethodofMEGA4.1.ThestatisticalresultsindicatedthatthelengthofITSsequencesofeachCoccomyceswasrangefrom436bpto457bptheGCcontentsoftheITSsequencesvaried

10、from48.0%to51.6%thelengthofITSsequencesGCcontentofintraspecificstrainsarelowervariablethanthatofinterspecificstrains.Thisshowsthatinterspecificgeicdiversityismeabundantthanofintraspecific.Aphylogeictreeof18strainswascons