19627.轉(zhuǎn)錄組數(shù)據(jù)注釋算法研究與軟件實現(xiàn)

1、中國科學院研究生院碩士學位論文轉(zhuǎn)錄組數(shù)據(jù)注釋算法研究與軟件實現(xiàn)姓名：劉遵明申請學位級別：碩士專業(yè)：生物信息學指導教師：于軍；吳佳妍201204ABSTRACT_________________—————————’——————_———————————●___________●_______________——__—_，—_____一______________________，_______一ABSTRACTWiththedevelopm

2、entofhigh—throughputnextgenerationDNAsequencing(NGS)technologies，RNAsequencing(RNA—Seq)hasbecomeoneofthemostimportantnewapproachesforbothgeneexpressionanalysisandtranscriptomesanalysisThemaincommercialavailableNGSplatfor

3、msareIllumina／SolexaGenomicAnalyzerRoche／454LifeSciencesGSFLXandABI／AgencourtSOLIDSystemNGStechnologyisalarge—scaleandhighthroughptttechnologywhichconsumeslessmoneyandtimeOntheothersideofthecoin，shortreadslengthcausesmor

4、ebioinformaticsbarriersWhenweobtainedRNA—SeqdatafromNGSplatforms，basicanalysisfollowstwosteps：mapRNASeqreadsteferencegenome，whichiscalledmapping；countreadsnumberanddensitycorrespondtoeachknownexon，spliceeventornewcandida

5、tegene，whichiscalledexpressionquantizationThemaincontentsofthispaperisthesecondstepofthesequencedataannalysis，whichiStheresearchofRNASeqdataannotationTheresearchcontenthavebeendevidedintofoutparts：First，weshouldtakealite

6、ratureresearchandgetthestudyofbackgroundknowledge，whichcanhelpUStounderstandthecurrentannotationtoolandanalysisofthestrengthsandweaknessesofthesetools；andthenconductresearchandanalysisofRNASeqdataannotationalgorithmandto

7、designthealgorithmofthiscomment，whichCanmeasurethegeneexpressioninisoformlevel；thereafterbasedontheshortcomingsanddeficienciesoftheexistingannotationtools，combinedwitltheannotationalgorithmdesignedbythesecondstep，wehaveb

8、eendevelopedanewRNA—Seqdataannotationtool；atlast，annotationalgorithmwehavedesignedshouldbeverifytodeterminetheaccuracyandavailabilityofresults，andweshouldtakeaappropriatetestofthannotationt001Fromwhatdiscussedabove，Wedev

9、elopatoolforRNASeqdataannotationnamedRNADAP(RNASeqdataannotationpipeline)ThispipelinecanevaluategeneexpressioninisoformlevelandbalancetimeconsumingandinternalstoragecostaswellMostimportant，ourpipelinecanbecompatiblewithm