18790.生長素和油菜甾醇在擬南芥根重力應(yīng)答中的相互作用分析

1、南開大學(xué)碩士學(xué)位論文生長素和油菜甾醇在擬南芥根重力應(yīng)答中的相互作用分析姓名：朱輝申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：植物學(xué)指導(dǎo)教師：門淑珍201205AbstractAbstractRootgravitropismisanimportantfeatureofplantstoadapttotheenvironmentItcanguidetheroottogrowalongthedirectionofgravityRootgravitropismisa

2、ctuallyacomplexphysiologicalprocessthatincludesgravitysensing，signaltransduction，asymmetricauxindistributionandgrowthresponsesThesiteofthegravitysensingiscolumella，andthesiteofgrowthresponsesisrootelongationzoneAuxinisth

3、eonlyplanthormonethathasthecharacteristicsofpolarVansport，anditsdirectionaltransportinplantsrequirestheinfluxcarrierAUX／LAXproteinfamilyandtheeffiuxcarrierPINfamilyandPGPfamilyThesecarriersarenecessaryforrootgravitropism

4、BRisiflsoinvolvedintheregulationofrootelongationandrootgravitropism，anditinteractswithauxinintheseprocessesButitisuncertainhowauxininteractswithBRduringrootgravitropismMutantstudiesshowthattherootelongationofbril一4、auxl—

5、Tandpin2一Taresignificantlyinhibited，but：det2一JrootelongationhasnodifferencewithwildtypeMutantauxlhasmoresevere‘gravitropismdefectsthanpin2，andauxl；pin2showauxlphenotypeingravitropism，whichshowAUXlplaysamoreimportantrolet

6、hanPIN2duringtheregulationofrootgravitropismWefoundthatexpressionlevelofBRll一∞decreasedinauxlandauxl；pin2buttheexpressionlevelofBRll一GFPhasnoobviouschangeThearticlealsopointsoutthatbril4showingweakrootgravitropismmaybeat

7、tributestothesefinds，forexample，thecellularexpressionlevelofPIN3inthecolumella，isreducedtheexpression1evelof刪2decreasedinepidermalandconicalcell，andtheexpressionpattemofAUXlisabnormalinthelateralrootcapandrootepidermalce