酶催化放大壓電生物傳感器的研究

1、湖南大學碩士學位論文酶催化放大壓電生物傳感器的研究姓名：申田田申請學位級別：碩士專業(yè)：分析化學指導教師：吳朝陽20090601酶僻化『】芟人j在I也傳感器的研究AbstractUseofbiosensortodetectthebiomarkersfortheaccurate、rapidanddiagnosisofclinicaldiseaseshasbeenanattractiveandhottopicinthecurrentmedic

2、alstudiesThequartzcrystalmicrobalance(QCM)isasimple，highspecificity，costeffective，freefromtracerlabeling，real—timedigitaloutputsensingtechnique，whichissensitivetomultiplesignalssuchasthesurfacemass，andthedensity，viscosit

3、y，conductivity，anddielectriceffectofthetestsolutionThetechniquepossessesawidedetectionrangeInthispaperincorporatinghighsensitivityofpiezoelectricresponseandbeingeasytoseveralpiezoelectricbiosensor，wedevelopedimmunosensor

4、andDNAsensorforthediagnosisofsomeclinicaldiseasesincludingSchistosomiasis，LpneumophilaetcThedetailedmatericalsaresho’Nnasfoolows：(1)AgaspiezoelectricquartsensorforsimultaneouslydetectionoftheantibodyofSchistosomaJaponicu

5、m(SjAb)wasdevelopedThisworkisbasedonenzymaticdepositionofsilverleadingtoamplifyfrequentcyshiftwhichislocalizedwheretheenzymaticlablegoatantirabbitIgGisattachedThesensorissimple，sensitiveandrapidInaddition，wealsoprovidean

6、ovelassaytoreaducethebackgroundbyusingcomplexingagentaswashingsolutionThedetectionlimitisobtainedaslowas1ng／mL(2)Ahairpinprobemodifiedwithathiolatits57endandabiotinatits37endwascombinedwithasandwich—typehybridizationassa

7、y，usingstreptavidinalkalinephosphataseasreportermoleculeThesensorissimpleandfrequentThedetectionlimitisobtainedaslowas045nmol／LThepresentedDNAbiosensorhasgoodselectivity，beingabletodiscriminateLpneumophilaanditsinterfere

8、ncewhichisdoublebasemismatches(3)UsingFe@AuascarriertheimmobilizationofMBwasmoreeffectivebecauseofgoodbiologicalcompatibilityofAunanoparticleMoreover，wealsodevelopedaeasilyreproducibleDNAsensorThedetectionlimitisobtained