畢赤酵母產(chǎn)木聚糖酶的發(fā)酵工藝研究

1、南京工業(yè)大學(xué)碩士學(xué)位論文畢赤酵母產(chǎn)木聚糖酶的發(fā)酵工藝研究姓名：賈偉申請學(xué)位級別：碩士專業(yè)：發(fā)酵工程指導(dǎo)教師：萬紅貴200804ABSTRACTABSTRACTSecondonlytocelluloseinnaturalabulldance，xyl孤wasam萄orcomponentofhemicellulose觸ctionoftlleplaIltcellwalls)(yl肌嬲eswerea伊oupofen巧mestllatbreal(d

2、ownxyl孤si11to)()r100ligosaccharides，Xylose鋤d鼬inose，鋤dthuswereofhi曲potentialforuseiIlfeed，papermal(iIlg鋤dfoodindus咄Ttlepaper，Thefementationconditionsforproducingxyl孤aseby尸f幽婦p儺fD，括wereiIlVestigatedThemaillcontentandresult

3、0fthestudywere勰following：1FromSinglefactorexperiInent，ttleoptim啪carbonsourceswaSbran，theoptim啪ni仃ogensourcesw懿ye笛tex艦ct，meoptirn啪s仃ainagesw鶴24h鋤dtheinoculatedVolumew弱1O％，t11eoptiInali11itialpHwaS55Intheoptimalfementation

4、conditionsmexyl鋤觴e∞tiVi夠infementationliquor化ached1600ⅣmL12ThemennostabilityofXyl鋤弱e舶m戶砌腸p儺f甜西w觴sn0ng，Theoptilll啪temperatll陀ofxyl肌弱ew弱70℃3AmodifiedfementationmediumcOmpositionw觴eXploited，mecheapye嬲thydrolysatew弱attemptedt

5、otaketheplaceofyeastex仃actduriIlgfe咖entationprocessTheOpt岫izedcOncentrationofyeaSthydrolysatew弱4％4Thehydrol婦ionpre仃eanllentiIltobr孤w弱usedtoinducetoeXpressmexyl趾弱emo他e行ectiVlyTheoptiInumdigestconcen廿ationw鶴20％The】(yl姐嬲e∞t

6、iv時reachedabout3000IUmL1afterthemediumw觴opthized5Theinfil仃atesofcells肌dbatchfementationiIl5literfermentorwe他iIlitial他searchedThexylan勰eactiVit)，w弱more吼h觚cedwhentlletween80w鶴addedtotllecultⅢemediu】[Il，tlletween80wasaddeda

7、Rer20hourS觚dthedensityw鸛02％ThemetllalloliIlductionoptimizedtimepointw硒l8hmexyl觚鶴eactiVityreached3479IU。mL叫aRertllemetll孤olintemlissionfeedingmodew嬲usedtoinducetoeXpresstllexyl鋤aseGlycerolfeedings仃ategieswasstudedIIlthisp

8、apertllefe肌entationtechnologyw觴established，whichwasutilizationofwheat髓dwaSteyeastforxyl鋤觴eproductionby尸￡幽砌p儺幻，西Comp盯atiVewimothertechnologyby尸f如婦p黜fD，括，theadVantagew弱也attllemedi啪w勰mo艙cheap，mexyl鋤鵲eactiVi夠w弱mo艙high，meappl