生物法合成1，3丙二醇的過程工程研究

1、——醉850‘6铘l⑧天洋大謦博士學位論文■_誓n●啊■■：塒習Ⅺ哪■■“I一級學科：化學工程學科專業(yè)：生物化工作者姓名：陳宏文指導教師：胡宗定方柏山教授天津大學研究生院2005年6月ABSTRACT1，3Propanediol(13PD)asanimportantchemicalproductcallbeusedaschainextenderforthesynthesisof1ublicantsolventandprecursorin

2、thechemicalandpharmaceuticalindustries，inparticularasamonomerforpolycondensationstOproducepolyesters(PTT)，polycthersandpolyurethanes1，3PDisproducedbytwomethods：chemicalsynthesisandmicrobialconversionBioconversionispartic

3、ularlyattractiveinthattheytypicallyuserenewablefeedstockanddonotgeneratetoxicbyproductsinthispaperpreparationofenzyme，processofenzymaticsynthesisanddesignofnonchargedultrafiItrationmembranereactorwerestudiedwiththeobject

4、ofdesigninganewbiosynthesismethodfor13PDproductionbyglyceroldehydrogenase(GDH)and1，3propanedioloxidoreductase(PDOR)fromKlebsieltapneumoniaeThemethodsofcelldisruptionanddeterminationofkeyenzymeactivityof1，3PDproductionwer

5、eestablishedunderaerobicconditionsAReroptimizationsofmediumbymethodsoftuffformdesign，artificialneuralnetworksandgeneticalgorithmsandfermentationconditions，theactivitiesofGDHandPDORwere3700and3840U／LGDHandPDORwerepurified

6、byionexchangeandaffinitychromatographytwo—stepsproceduresimultaneouslyunderaerobicconditionsWhencellsofKlebsiellapneumoniaewereculturedinbuffersupplementedsemicarbazideandglycerol，aerobicconversionofglyceroltO3hydroxypro

7、pionaldehyde(3HPA)realizedAmethodbasedonHPLCfortheanalysisofglycerol，3HPA，13一PDanddihydroxyacetone(DHA)simultaneouslywagestablishedStudiesontheinitialvelocityandproductinhibitionofGDHandPDORwereconsistentwithanorderedBiB

8、imechanismandthekineticmodelsofGDHandPDORwereestablishedrespectivelyUnderaerobicbatchoperationcondition，coupledreactionwithNAD(H)regenerationforproductionof1，3PDandDHAwaspossibleOptimumretentionofnativeNAD(H)innoncharged

9、ultrafiltrationmembranereactor“wasinvestigatedTheproductionof1，3PDbyKlebsieltapneumoniaeinNaCSPDMDAACmicrocapsuleswasstudiedTheencapsulatedcellswerefermentedsequentially10batchesinshakingflasks，themaximum1，3propanediolpr