以油醇為高效萃取劑的丁醇萃取發(fā)酵和回收萃余液中溶劑產(chǎn)物的研究

1、江南大學(xué)碩士學(xué)位論文以油醇為高效萃取劑的丁醇萃取發(fā)酵和回收萃余液中溶劑產(chǎn)物的研究姓名：牛曉樂申請學(xué)位級別：碩士專業(yè)：發(fā)酵工程指導(dǎo)教師：史仲平20080801AbstractAbstractThemainproductsofbutanolfermentationwereacetoneandbutan01Thefermentingcharacteristicofhighbutanol／lowacetonewasgoalforpeopleIt

2、dependedontheintracellularreducingpowerwhethertheproductwasacetoneorbutan01TheproductionisbutanolwhenintracellularreducingpowerWasstrongIntraditionalfermentation，01％(w／v)neutralredorlOg／Lsorbitolor8mg／LnicotinicacidWasad

3、dedinthemediumtostrengthenthereducingpowerItWasfoundthatcomparedwitlltraditionalbatchfermentationthegasproductionperliterfermentationliquidlowerby5L，thebutanolconcentrationincreasedby184％andtheacetoneconcentrationdecreas

4、edby213％whenadding01％(w／v)neutralredtothemediumThesolventproductofbutanolfermentation，especiallythemainsolventproductofbutanolWasatypicalmetabolicinhibitionproductTheextractivefermentationwimoleylalcoholcouldenhantethepe

5、rformanceofbutanolfermentationasitcouldavoidinhibitionNeutralredcouldpartiallyreplacedFdtotransportelectronandprotonItforcedFdredtosupplementNADHbyreducingNADwhichstrengthenedthereducingpowerThecombinationofusingoleylalc

6、ohosasextractantandaddingneutralredaselectroncardercouldfurtherimprovethefermentationperformanceundertheconditionofusing15％compowdermediamAttheendoffermentation，thebutanolconcentrationandtheproductintensityrespectivelyin

7、creasedby452％and245％，comparedwitllextractivefermentationTheextractivefermentationwithbiodieselastheextractantcouldgettingimprovedbiodieselwithbutanolafterfermentation，whichcouldbeuseddirectlyandleavedouttheenergyforcolle

8、ctingbutan01Butintheaboveprocess，itproducedalotofextractionraffinateIftheextractionraffinatecouldbeusedtomixmaterialsaswaterinnextfermentation，itcouldeconomizelotsofwaterButtherewerealsotwoproblemsinthetreatmentforextrac

9、tionraffinateOneWasthehighbutanolconcentrationinitwhichinhibitedusingaswatertomixmaterialinthenextfermentationTheotherWasalotofsolventproductinunrecoverableraffinateThecostofdistillationpurificationcouldreducegreatlyifth

10、ebutanolinvolvedintheextractionraffinateWasrecoveried、航mtheeffectiveextractantoleylalcoholwhichcouldincreasethebutanolconcentrationinoleylalcoh01However，theextractant—oleylalcoholisprecious，althoughitmayberecycled，itsamo

11、untofuse(cost)isamajorproblemSotherepeatedextractionandmultistageextractionwitholeylalcoholWasstudiedbycomputersimulation，andtheoptimizationWasgotUndertheconditionofbutanolconcentrationininitialextractionraffinatewaslOg／