特異性PCR在檢測玉米ae基因中的應(yīng)用.pdf

1、甘肅農(nóng)業(yè)大學(xué)碩士學(xué)位論文特異性PCR在檢測玉米ae基因中的應(yīng)用姓名：雷武生申請學(xué)位級別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：王漢寧20050601SummaryMaizeisoneofthemostimportantcropplantsintheworldandcornstarchisamainstarchsourceofhumanbeingThestarchisamainbacklogmaterialofthecornseed，thema

2、izestarchisabout70％oftheseedThestarchexistsintwoforms：AmylopectinandamyloseThecommoncornstarchisamixtureofAmylopectinandarnyloseandtheircontentisabout28％and72％respectivelyBecauseofitsspecialphysicalandchemicalproperties，

3、anaylosestmchisakindofhighadditionalvaluestarchAmylosestarchisarlimportantindustrialmaterialandappliedinextensivelyinmorethan30fieldstlowever，theamylosecontentincommonComisratherlowgenerallybetween22％28％Thislimiteditsapp

4、licationinindustrybecausethecostofseparatingthehighanwlosefromthecommoncornisveryhighThus，itisnecessaryforcornbreederstoexplorehighamylasecorntofitforindustrialuseThebreakthroughofhighamyloseresearchisthediscoveryoftheae

5、gene(amylaseextender)mutationbodytheamylosecontentsraisedconsumingwithaegeneAfterwardsothermodilyinggenesarediscovered，theyaffecttheratioofamylopectinandamylosetnthecurrentresearch，22pairsofspecificprimersweredesignedacc

6、ordingtotheaegenesequencepublishedinGenbankThreeinbredlinesofcommoncordandthreeaemutantinbredlinesandthreematerialsofhybridlinesweremarked，throughpolyacrylamidgeleletrophoresisdetecting，theresultasfollowir／g：(1)Primer4、7

7、、t0、11、15andl8didnotshowanybandsinthemateriall9(2)Primer1、12、14、16、17and19showedtargetbandsinthemateriall一9，thebandshavenodiflorence(3)Primer3、6、20and21showedmanybandsinthematerial19，itcontainedtargetbandsandotherunspeci

8、ficbands(4)Primer2showedmanybandsinthematerial19，butitisnotcontainedtargetbands(5)Primer8、9、13and22showedtargetbandsinsomematerialandthebandshavenorule(6、Primer5showedtargetbandsinmaterial1、2、4、5、7、8andhavenobandsinmater

9、ial3、6、9Theprimer5showedstrongspecialtyusingprimer5todetect21hybridlines(materiall130)Primer5showedtargetbandsinthehybridofcommoninbredlinesandaematerial(material11、12、13、14、15、16、17、18、1920、21、22、23、24、25、26)havenobands

10、incomlTlOnhybrid(material27、28、29、30)ItisconcludedthatthispairofprimeroandistinguishhybridlinesAeAefromhybridofhighamylosemaizeAeaeItisaneffectivetechnologytoimproveinbredlinesincommonusetoinbredlinesofhighanaylosemaizeK