用花培法輔助選育辣椒雄性不育系和恢復(fù)系的研究.pdf

1、中國農(nóng)業(yè)科學(xué)院碩士學(xué)位論文用花培法輔助選育辣椒雄性不育系和恢復(fù)系的研究姓名：邢永萍申請學(xué)位級別：碩士專業(yè)：蔬菜學(xué)指導(dǎo)教師：李錫香蔣鐘仁20050601AbstractF1hybridsaregenerallymorevigorousanduniformthanopenpollinatedcultivarsinpeppenTheyareusedwidelyinpepperproductionMalesterilityinpepperhas

2、beenstudiedformorethantwentyyearsinordertoimprovetheseedquailtyanddecreasetheproductioncostinChinaMuchprogresshasbeenmadeinresearchofnucleusgeniemalesterility(GMS)andcytoplasmicmalesterility(CMS)Butfertilerestorergenesof

3、pepperusuallyexistinvaluelessvarietyorwildgermplasmsTherearenohybridsdevelopedbymeansofCMSinsweetpeppersofarTherearethreegoalsinthisresearchThefirstistoobtaingenicmalesterilelinesthroughacombinedmethodofantherculture，cro

4、ssingandselfingThesecjndistotransferCMSfromonetoanothervarietybyanthercultureandbackcrossThethirdistoimprovefertilerestorerlinesassistedbyanthercultureForthefirstgoal，thefertileplantsingeniemalesterilelineswasusedasfemal

5、eparents，crossedwithanothergoodlines，andthentheFlantherwasculturedThemalesterileandmalefertileplantswithsimilarcharactersderivedfrompollencultureweremarkedandcrossedeachotherTheindividualfruitsfrommalesterileplantssimila

6、rtomale—fertileplantswereharvestedandgrownfornextgenerationinthesameyearBackcrosswasmadeagainbetweenmalesterileplantsandfertileplantswiththecharacterssimilartorecurrentparentSelfing，siblingcrosingandselectionwereperforme

7、dfor6generationsin3yearsThegeniemalesterileline(Qa18AB)wasobtainedThesegregationratiois1：1ofmalesterileplantsandfertileplantsThegeniemalesterilelineisuniforminmorphologicalcharacterForthesecondgoal，3FIhybridsproducedbyme

8、ansofCMSlineswereintroducedItsantherwasculturedAlotofmalesterileplantsandfertileplantswereobtainedTheelitemalesterileplantswereselectedfromanthercultureandweresuccessivelybackerossedrespectivelywithmalefertilelinesderive

9、dfromanthercultureandeliteinbredlinesasrecurrentparentThreemalesterilelines(m一19A，M22AY一56A)weredevelopedbyanthercultureofFlhybriddevelopedbyCMSlinesThesterilepercentageofthisthreesterilelinesis976％～100％Forthethirdgoal，t

10、heanthercultureof3F1hybridswasmadeThemalefertileplantswithgoodfruitcharactersfromanthercultureweretestcrossedwithmalesterileplantsfromantherculturemadintroducedmalesterilelinesEightfertilerestorerlineswereobtained，andthe

11、percentagesoffertileplantsallreach100％Threedevelopedrestorerlineswerecrossedwithelitelinesfromantherculture4sweetpepperfertilerestorerlineswithsquarelanternfruitand3fertilerestorerlineswithsheephemshapedfruitwereobtained

12、bymeansofFlsanthercultureThepercentagesoffertileplantsintest—CROSSprogenyis100％Inaword，thetimetodevelopgenicmalesterilelinesandcytoplasmicsterilelineswasshortenbycombinationofanthercultureandconventionalbreedingmethodThe