甘藍(lán)型油菜株高和開(kāi)花時(shí)間的QTL定位及黃籽性狀的分子標(biāo)記.pdf

1、中國(guó)農(nóng)業(yè)科學(xué)院博士學(xué)位論文甘藍(lán)型油菜株高和開(kāi)花時(shí)間的QTL定位及黃籽性狀的分子標(biāo)記姓名：梅德圣申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：作物遺傳育種指導(dǎo)教師：王漢中李云昌20041225AbstraetOilseedrapeisanimportantoilcropplantedextensivelyintheworldPlantheight，floweringtimeandseedcoadcolourplayimportantrolesinlodging

2、resistance，yieldformationandseedqualityrespectivelyResultsobtainedfrompreviousstudiesonthoseth糟etraitseouldn’tmeetthepresentbreedingdemandInthisstudyaBrassicanapusmutantlinewithdwarfandearlyfloweringcharacteristicswasuse

3、dtostudytheinheritanceofplantheightandfloweringtime，andtoidentifyquantitativetraitloci(QTLs)ofthesetwotraits；AnFzpopulationfromaCROSSerayellowseededandablackseededlineswasusedtoscreenmolecularmarkerstightlylinkedtoyellow

4、—seedgenes；Futhermore，ayellow—seededdoubledhaploid(DH)populationwasestablishedforgeneticsandmolecularstudiesofyellowseedcharacterThemainresultsaresummarized“following：1AhighstalkandlaterbloomBrassicanapusline2091wascross

5、edwithadwarfandearlybloommutantline99CDAM。Geneticanalysison&，F(xiàn)2，BCIpopulationsindicatedthatthedwarftraitw鸛controlledbythreepairsofreeessivegenes，withmaternaleffect，Earlyfloweringwasmainlycontrolledbyonepairofrecessivegen

6、esTherewassignificantcorrelationbetweenplantheightandfloweringtimefr=O2194“)2Alinkagemapwasconstructedfromasegregatingpopulationwitht45F2：sfamilylinesderivedfromthecrossof99CDAM2091Itwasconsistedof199AFLPand34SSRlociin20

7、mainlinkagegroupspluseightmarkersinfourlinkagepairscovering20946cMofthegenome。3WhenF2：；familylineswereusedtomapQTLsofplantheight，oneQTLonlinkagegroup(∽)I3wasidentifiedbysinglemarkeranalysismethodFiveQTLswereidentifiedbyb

8、othofintervalmapping(IM)andcompositeintervalmapping(CIM)methodsTheselociaccountedfor101—264％(1M)and113286％(CIM)ofphenotypicvariationrespectively4WhenF2，3familylineswereusedtomapQTLsoffloweringtime捃。QTLswasidentifiedbysin

9、glemarkeranalysismethod1MandCIMmethodsbothresultinfourQTLsTheselociexplained89304％ofphenotypicvadationWhenphenotypesofF2singleplantswereusedtodetectQTLsoffloweringtime，acommonQTL研13)wasfoundbyallthreemethodsIMmethodalsoi

10、dentifiedoneQTL0nLG3。whichaccountedf醅9，8％ofphenotypicvariationAnotherQTLonLG6with9，5％contributionratewasdetectedbyCIMmethodIntotal，fiveQTLsrelatedtoflowedngtimewereidentifiedintwopopulationsbythreemappingmethods5TheQTLma

11、ppingresultsshowedthatmainQTLsofplantheightandfloweringtimewerebothmappedonLGl3，andtheirQTLcreditableregionswereoverlapped。QTLsofplantheightandfloweringtimewerebothfoundonLG6，howevertheywerenotcloselylinked6DNAsamplesfro