肝癌多基因啟動子區(qū)域異常甲基化與預(yù)后關(guān)系的研究.pdf

1、安徽醫(yī)科大學(xué)碩士學(xué)位論文肝癌多基因啟動子區(qū)域異常甲基化與預(yù)后關(guān)系的研究姓名：彭曉春申請學(xué)位級別：碩士專業(yè)：外科學(xué)（普外）指導(dǎo)教師：耿小平朱立新20100401安徽醫(yī)科大學(xué)碩士學(xué)位論文彭曉春2009年5TheRelationshipBetweenAberrantPromoterMethylationofMultipleGenesPrognosisinHepatocellularCarcinomaAbstractObjective:Tost

2、udytherelationshipbetweenthegenepromotermethylationstateofDAPK、FHITSLIT2genestheclinicalpronosisofpatientinhepatocellularcarcinoma(HCC).Methods:ThetechniqueofmethylationspecificPCR(MSP)wasadoptedtoinvestigatethepromoterh

3、ypermethylationofDAPK、FHIT及SLIT2genesin50HCCsafteracurativeresection.Therelationshipbetweenthefrequencyofhypermethylationofthegenestumrecrudescencedatawasanalyzed.Results:InallpatientswithHCCthefrequencyofhypermethylatio

4、ninDAPK、FHITSLIT2were82.0%(4150)68.0%(3450)54.0%(2750)respectively.Wedividedallthosecasesintotwogroupsaccdingtothefollowupneoplasmrecurrenceresults.inthegroupofoneyearwithouttumrecrudescencethefrequencyofhypermethylation

5、inDAPKFHITSLIT2were76.9%（2026）、53.8%(1426)、50%(1326)respectivelyinthegroupoflessthanoneyearwithtumrecrudescencewere87.5%（2124）、83.3%（2024）、58.3%（1424）respectively.Thosethreegeneshavehigerfrequencyamongthegroupoflessthano

6、neyearwithtumrecrudescenceThefrequencyhypermethylationofFHITgeneisespeciallyhigherinthegroupoflessthanoneyearwithtumrecrudescence（p＝0.036）.Inthegroupoflessthanoneyearwithtumrecrudescencethereistwocaseswhichhasonenonegene

7、hypermethylationthereistwentytwocaseswhichhavetwothreegeneshypermethylationinthegroupofoneyearwithouttumrecrudescencethecasesareelevenfifteenrespectively.Thereisastatisticalprognosisdifferencebetweenthosecaseswithtwothre