健脾理氣中藥對肝癌及轉(zhuǎn)移影響的實驗研究.pdf

1、復旦大學碩士學位論文健脾理氣中藥對肝癌及轉(zhuǎn)移影響的實驗研究姓名：成文武申請學位級別：碩士專業(yè)：中西醫(yī)結(jié)合臨床指導教師：宋明志20031105AbstractObjective:Tostudytheefectofstrengtheningandregulatingspleenqirecipeonbepatocellularcarcinomaanditsmetastasisandtodetecttheinhibitingmechanismo

2、fgrowthandmetastasisofhepatocellularcarcinomabystrengtheningandregulatingspleenqi.Methods:33nudemiceweretransplantedinprimarysituswithHCCLM3ofmetastatichumanhepatocellularcarcinomarandomizedinto4groups.2groupsweremadespl

3、eenqideficiencymodel.(1)GroupA(TCMgroup):8nudemiceperfusedbyJianPiLiQi(strengtheningandregulatingspleenqi)decoction0.3mlpermouseb.i.d.(2)GroupB(spleenqideficiencynormalsaline):9nudemicewereperfusedDahuangMangxiao(rhuberb

4、andmirabilite)mixture0.3mlq.o.dandnormalsaline0.3mlb.i.d.(3)GroupC(spleenqideficiencyTCMgroup):8nudemicewereperfusedbyDahuangMangxiaomixture0.3q.o.dandJianPiLiQidecoction0.3mlpermouseb.i.d.(4)GroupD(controlgroup):8nudemi

5、cewereperfusedbynormalsaline0.3mlpermouseb.i.d.Allmiceweresacrificed37daysaftercancertransplanted.Theserumconcentrationofvascularendothelialgrowthfactor(VEGF)wastestedbyenzymelinkimmunoadsorbentassaysystemmethodTheexpres

6、sionofVEGFandmatrixmetalloproteinases2(MMP2)oflivertumortissueandlungmetastasestissueweretestedbyimmunohisochemistrymethod.Besidesthemicebodyweighttumorweightnumberofceliacmetastasesandlungmetastasessignsandsymptomsofmic

7、ewerealsoobserved.Result州1)Theaveragebodyweightsofthe4groupswereGroupA(23.0011.89)gGroupB(21.51士1.63)gGroupC(24.06土1.91)gGroupD(21.44士0.96)grespectively.ThediferencesinthebodyweightbetweentheGroupCandGroupBaswellasGroupD

8、werebothsignificant.(P0.01)(2)TheaveragetumorweightsofthefourgroupswereGroupA(3.15土1.27)gGroupB(3.88土1.27)gGroupC(2.30士1.61)gGroupD(4.00士1.11)grespectively.ThediferencesinthetumorweightbetweentheGroupCandGroupBaswellasGr