木霉制劑防治蔬菜土傳病害和gfp基因轉(zhuǎn)化的研究.pdf

1、浙江大學碩士學位論文木霉制劑防治蔬菜土傳病害和gfp基因轉(zhuǎn)化的研究姓名：李靜申請學位級別：碩士專業(yè)：植物病理指導教師：徐同張敬澤20070501AbstractSoilbornediseasesofvegetablesweredistribmedovertheworldandcallcauseseveredamageBiocontrolhavebeenprovedtobealleffectivealternativetomanageth

2、iskindofdiseaseMoststudieshavefocusedonbiocontrolofsoilbornediseaseswithTrichodermasppandmanyTrichodermaproductswerecomercializedathomeandabroadHowevershortshelvetimelimitedtheirpraticaluseMoreovermostofthemalesingleTric

3、hodermastrainproducts，whichisnotsteadyintheapplicationInourpreviousstudiesfharzionumstrainTC3andNFgZvirensstrainTY009haveshowedantagonisticeffectonsoilbornepathogensThebiocontrolpotentialofthemixtureagentsofthethreestrai

4、nsofTrichodermaagainstvegetablesoilbornediseasesWasevaluatedinthisstudyThegrowingconditionsofmixturedTrichodermastrainswerealsoevaluatedindifferenttreatmentsItisshowedthatitWasgoodformycelialgrowthandsporegerminationatth

5、eration6：4(W：W)ofwheatbranandricehuskriceBranandricehusk；atration4：6(W：W)ofwheatbranandricericebranandriceInaddition，watercapacityinsubstratebetween12：1～14：1andcontrotedlight12hblack12hWasthebestforgrowthofsporemixtureso

6、fTrichodermaThebiocontrolefficacyofthesporemixturesofTrichodermaingreenhouseWasalsodeterminedTheresultshowedthatdampingoffofcucumbers，dampingoffofeggplants，sclerofiumdiseaseoflettuce，fusariumwiltofcucumberandblackshankdi

7、seaseoftobaceoweresuppressedby755％550％59O％TheresultsalsoindicatedthatthebettercontrolforfusaliumwiltofcucthnberandblackshankdiseaseoftobaccoInadditiomthemixtureagentofTrichoderl“tashowedbettereffectoncontroloffusariunlwi

8、ltofcucumberthatthatofsingleapplicationwith3Trichodermastl面lasaloneInordertoobservecolonizationofthemixtureagentofTrichoderma，weconstructedthevectorscontaining加geneforitsexpressioninTrichodermastrainandthetransformationo