小麥品種偃展2號抗白粉病基因的SSR標記.pdf

1、河北農(nóng)業(yè)大學碩士學位論文小麥品種偃展2號抗白粉病基因的SSR標記姓名：曹乃倩申請學位級別：碩士專業(yè)：作物遺傳育種指導教師：劉桂茹楊學舉20070607TheStudyonSSRMarkersLinkedtoPowdery]Ⅷ[IdewResistanceGenesInWheatYanZhan2ItaoAuthorCaoNai‘#uMajocCropOcnefirta叫BmdingSupervisor：FrofesmrL抽Guimprof

2、essorYangXue]ePowderymildewcausedbyErysiph己graminisfspTtriaciisadestructivediseaseofcomillonwheatthroughouttheworldInviewofhi窖hgeneticvariabilityofErysiphegraminisfspTriticiidentifyingresistantgenesandusingittobreedresis

3、tantcultivarsisthemosteconomicalandeffectiveapproachtoconffolthisdiseaseThereforetheexploitationofPowderymildewresistantgeneswh／chincludediIsmarkinglocationandmolecularmarkeraidedselectionwouldbenefitbreedingtheeffective

4、PowderymildewresistantcoltivarsInthisstudythePowderymildewresisrantgeneofvarietyYanZhan2HaowasanalyzedbyusingSSRtechnique1nlcresultsa聆shOWedjustasfollows：1CrossWSScardedoutbetweenYanZhan2Hao85resistantparentandCangMm030a

5、ssusceptibleparentandFtplantsfromthetaDSSwereselfedtoobtainF2populationInF】population，everyindividualhadresistancctOPowderymildewButF2populadonwasdividedintotWOkinds：resisrantindividualandsuscepfiveindividuaLtheratiobetw

6、eenresistantindividualandsusceptiveindividualaccordedwith3：1GeneticanalysisCOilcludedthattheresistanceWasCOntrolledbyapairofsineledominantgone2BulkedsegregantanalysisOjSA)Wasusedtoestablishresistantandsusceptivegenepooli

7、nordertosearchforSSRmarkerswithpolymorphismThePCRresultsamplifiedbySSRprimersoftheseF2individualswereanalyzedbyusingMapmaker／EXP(VERTION30b)andMapmanagerTheresultsindicatedthatthisgeneWaslocatedODthechromosome2D3Thegenet