新型有機(jī)物料腐熟劑中微生物種群組成及作用機(jī)理的研究.pdf

1、Il王07681分類號密級。專嚼差≮夭等。碩士學(xué)位論文單位代碼：學(xué)號：10019S040667新型有機(jī)物料腐熟劑中微生物種群組成及作用機(jī)理的研究Microbialcommunityanditsfunctioninaorganicmatterdecomposinginoculant研究指導(dǎo)教生：韭嬙師：袁紅麴麴援申請學(xué)位fl類級別：堡堂亟稱：邀生物堂院：生物堂院2007年6月名方學(xué)業(yè)究在專研所AbstractOrganicmatterde

2、composinginoculantisakindofmicrobialinoeulantswhichcouldfermenttheorganicmattertoproducebiologicalorganicfertilizerThenewstylemganJcmatterdecomposinginoeulantisusuallyacomplexofmicrobesIthasagreatimportanceinusingagricul

3、turalresoure2andprotectingenvironmenteffectivelyInthisstudyweresearchedonthetwokindsoforganicmatterdecomposinginooulant(AandB)whichwereobtainedfromnaturebyourlaboratoryandinvestigateditsmicroorganismcompositionanditsfuoe

4、tionmechanismtoprovidethetheoriesbasisforthereasonableusingoforganicmatterdecomposinginocoiantTwentyouebacteriafromthesampleAthirtysevenbacteriatwoyeastsandol碡fungiwereisolatedfromthesampleBTheactivitiesofextracellularen

5、巧m髂oftheisolatedbacteriawerestudiedAmajorityofstrainscouldexcreteextracellularamylaseandprotease，andthehighestamylaseandproteaseactivityreached3324U／mLand1202U／mLrespectivelyAfewstrainbacteriacouldexcretecellulosemadurea

6、sewRhthehighestactivityreachedO145U／mLand1656U／mLrespectivelyThemicrobecommunitycompositionoftheorganicmatterdecomposinginoculantwereaaalyzodFourtytwobacteriastrains’16SrDNAPCRproductsWergdigestedbytworestrictionenzymes(

7、HinfIandCspoDandtheresultsshowed11kindsofARDRAtypestotallyexpressingto11genotypesThebacteriarespectivelybelongtoPcmtoeaagglomeransstrain、Enterobacteriaceaebaeteri啪v429、BacillussubtiffsstrainCGMCCl869Bacillussubtillsstrai

8、nCICCl0028、Bacillusamyloliquefaciens、Enterobactersakazakii、AcinetobacterbaumannfjstrainAb8、Bacillusmegaterium、Weissellacibaria、KlebsiellapneumoniaeandPantoeaananatisstrain3404，Inaddition，genomicDNAofthebacterialstrainswe

9、reamplifiedbyPCRwithspecificprimersOfERICsequence，acquiring23kindsofdifferentfingerprinttotallyBycomparisonoftheresultsobtainedfromthe16SrDNAclonelibraryandtheplatecultureisolationitcouldbeindicatedtlIatthedominantclonei

10、nthelibraryandthestrainsfromthepureculturehaveahigllsimilarityinphylogenesisFurthermore，theclonelibrarycontainedthebacteriawhichwerenotisolatedbytraditionalculturingapproachThissuggestedthatthebacterialdiversitystudiedby

11、16SrDNAclonelibrarymethodisricherthanthatoftheculturalapproachThe16SrDNAclonelibrarymethodcouldbe柚effectivetechniquetostudythemicrobecommunitycompositionoftheorganicmatterdecomposinginoculant11櫓antagonismbetweenisolateds

12、trainswerestudiedAccordingto1lkindsofARDRAtypesselectedtherepresentativebacteriastrains、yeastsandfungitotesttheirgrowthrelationrespectively1heresultssuggestedthatantagonismexsitsinsomemicroorganismsAppliedthemicrobialfer

13、mentationcommunityintothefermentationexperimentThewaterandtemperaturevarietysituationduringtempestingandseedgerminationresultsshowedthemixedmicrobialfermentationcommunityandtheorganicmatterdecomposinginooulantcanhavesimi