炭疽桿菌A16與A16R比較基因組學(xué)研究.pdf

1、中國人民解放軍軍事醫(yī)學(xué)科學(xué)院生物工程研究所碩士學(xué)位論文炭疽桿菌A16與A16R比較基因組學(xué)研究姓名：何湘申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：朱厚礎(chǔ)黃留玉20040530ComparatiVeGenomicsbetween艿口c掰配s口n踴mc婦A16andA16RAbstract。如cj』u占a門六慨cjStheetiologicalagentofanthrax，isresponsibleforaseriousandoftenfa

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3、microbiology丑a門廳憎cjsA16RisattenuatedfromA16byProfessoryangshuyain1953Suppressivesubtractivehybridization(ssH)isaPCRbasedDNAsubtractionmethodthatenablestheidentificationofgenomicsequencedifferencesbetweenvariousstrainsors

4、peciesofbacteriaForexample，SSHhasbeensuccessfullyusedtoidentifyuniquegenomicsequencesforspecificstrainsof脅』fcD6ac芒cir。盧_yDrLuropathogenic凸c廳∥f拍如cD』j，andc010nizing如印rZ。如cf盯地在坍Thistechniquehasalsobeenusedtodetectuniquegeno

5、micsequencesfoundinEcoiibutnotlnSalmonellatyphimuriUm，lnpatho《enic8urkhoideriapsPudb月魎，』Pj‘butnotinnonpathogenic廈西af』a力幽門sj占，inpathogenicBurk南。ideriamaiIeibutnotin87thajandt明sjsandinBan拍racisbutn。t1nBcereus1451191，Bthuri

6、ngiensisAHakamorBcereus3～，1his【nethod0109yhasn。tonlyidentifiedgeneticdifferencesamongdifferentstrainsorspeciesofbacteriabutalsodefinedgenesthatcontributetotheVirulenceofan0rganis皿BythemeansofSSH，thedifferencebetween歷。j』』