傳瘧媒介大劣按蚊和斯氏按蚊基因組DNA遺傳多態(tài)性研究.pdf

1、安徽理工大學碩士學位論文傳瘧媒介大劣按蚊和斯氏按蚊基因組DNA遺傳多態(tài)性研究姓名：孫恩濤申請學位級別：碩士專業(yè)：病原生物學指導(dǎo)教師：秦志輝20070601安徽理工大學碩士學位論文AbstractObjeefiveToanalyzethegenomicDNApolymorphisminAn6矗rusandAnstephensiofdifferentsusceptibletoPlasmdiumyoelii，SOastoexplorehost

2、genotypebyparasitegenotypeinteractionsunderlyingthesusceptibilityofanophelinemosquitoestoplasmodiumMethodsThegenomieDNAwasextractedfromtheadultsofAndimsandAnstephensiOntheonehand，thegenomieDNAforbothspecieswereamplifiedb

3、yRAPD，andthentheamplifiedproductswerecarriedonagaxosegelelectrophoresisThegelpurifiedRAPDbandswhichhadsimilarmobilityWCleisolatedandsequencedSequencealignmentwasperformedbyusingDNASISprogramOntheotherhandtherDNAITS2regio

4、nforbothspecieswe糟amplifiedbyPCRandsequencedandtheamplifiedproductswereanalyzedbyrestrictionendonucleasedigestion(RFLP)ThecorrespondentsequenceofAn西h口andAnstephensiweresearchedfromGenBankAllthesesequenceswe∞alignedandcom

5、paredusingClustalWprogramandthephylogenetietreewasconstructedbyMEGAprogramResultsThegenomicDNAofAndiruaandAnstephensiweresuccessfullyamphfiedbyRAPD，andtherewasobviousdifferenceintheirRAPDfingerprintingongenomieDNArespect

6、ivelySequencealignmentoffourpairsofRAPDbandswhichhadsimilarmobilitybetweendifferentanophelinemosquitoesshowedthateachpairofRAPDbandsWOIedifferedinGCcontentandSimpleSequenceRepeatandlheirdifferenceWel“einthearrangefrom600

7、％to619％innuclentidesequencesItwasdemonstratedthattherewerepoly】morphisminthelengthandconstructionofRAPDbandswhichhadsimilarmobilityMoreoverthere撇significantlydifferentfromtheRFLPpatternsofrDNAITS2inAndimsandAnstephensiby

8、restrictionendonueleaseXhoLthelengthofITS2regionwas715bpinAn舭and466bpinAnstephensiSequencealignmentofITS2regioninAndirusandAnstephensishowedthatITs2regionWCl“edifferedinGCcontentandSimpleSequenceRepeal，andthedifferencewa