UV-B輻射增強(qiáng)對(duì)水稻根系活力及其蛋白質(zhì)組的影響.pdf

1、福建農(nóng)林大學(xué)碩士學(xué)位論文UVB輻射增強(qiáng)對(duì)水稻根系活力及其蛋白質(zhì)組的影響姓名：胡安生申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物工程指導(dǎo)教師：林文雄黃顯波20081201福建農(nóng)林人學(xué)碩士學(xué)位論文UVB輻射增強(qiáng)對(duì)水稻根系活力及其蛋白質(zhì)組的影響摘要通過對(duì)Uv—B輻射脅迫下水稻根系響應(yīng)的研究表明：Uv—B輻射對(duì)供試兩個(gè)水稻品種的根系活力和發(fā)根勢(shì)均有顯著提高，進(jìn)一步對(duì)根系蛋白組的2DE蛋白電泳圖譜分析發(fā)現(xiàn)在UV—B輻射處理下Lemont發(fā)現(xiàn)23個(gè)蛋白差異點(diǎn)，Du

2、lar發(fā)現(xiàn)25個(gè)蛋白差異點(diǎn)，從已有的根系肽指紋圖譜庫(kù)中鑒定出8個(gè)Lemont品種的差異點(diǎn)蛋白、6個(gè)Dular品種的差異點(diǎn)蛋白。這些蛋白可能與水稻對(duì)Uv—B輻射脅迫的響應(yīng)有關(guān)。關(guān)鍵詞：水稻Uv—B輻射雙向電泳根系活力蛋白質(zhì)組Effectofenhancedultravi01et—Bradiationonthe●●●‘●●rootactlVltyand1tSproteomiCS1nrlceAbstractThechangesofrootac

3、tivityanditsproteomicsintwodifferenttestedricecultivarsexposedtoenhancedultraviolet—BwereinvestigatedTheresultsshowedthatUV—BradiationstressincreasedtheactivityandgrowthoftherootsystemsintwicecultivarsAccordingtotheanaly

4、sisofthe2一DEgelinproteomicsoftherootsystems，23differentproteinspotsinLemontwerefoundunderenhancedUVBradiationand25differentproteinspotsinDularAnd8differentproteinspotsinLemontand6inDularwasidentifiedfromthethePeptidefing