Twist-1介導的衰老阻滯在宮頸癌發(fā)生與發(fā)展中的作用機理.pdf

1、華中科技大學碩士學位論文Twist-1介導的衰老阻滯在宮頸癌發(fā)生與發(fā)展中的作用機理姓名：陶濤申請學位級別：碩士專業(yè)：婦產(chǎn)科學指導教師：王世宣2011-05華 中 科 技 大 學 碩 士 學 位 論 文 華 中 科 技 大 學 碩 士 學 位 論 文 2Role of Twist-1 mediated senescence in the process of occurrence and development of cervical c

2、ancer Abstract Objective : To Investigate the role of Twist-1 mediated senescence suppression in the process of Cervical Intraepithelial Neoplasia (CIN) developing to cervical cancer, by detecting the expression of Twist

3、-1 2. The expressions of Twist-1 and CBX-3 among groups were detected by immunohistochemistry (SABC) method; 3. Specific Twist-1 small interfering RNA (siRNA ) were transfected in SIHA cell of human cervical cancer cel

4、l line to down-regulate the expression of Twist-1 and sequentially identify the state of senescence using SA-β-gal staining. Result: 1. During the process of normal cervix developing to cervical cancer, the expression

5、of Twist-1 was gradually increased. 2. The expression of CBX-3 was increased simultaneously with development of CIN, and it was at the peak in the CIN III, but significantly decreased in the state of cervical cancer form

6、ation. 3. Being confirmed in vitro test of SIHA cell, the senescence of SIHA cell can be induced by down-regulating the expression level of Twist-1. Conclusion: The Twist-1 mediated senescence suppression plays an antit