賴氨酸修飾的交聯(lián)殼聚糖微球共固定化GOD-CAT的研究.pdf

1、重慶大學(xué)碩士學(xué)位論文賴氨酸修飾的交聯(lián)殼聚糖微球共固定化GODCAT的研究姓名：蘇霞利申請學(xué)位級別：碩士專業(yè)：化學(xué)工程與技術(shù)指導(dǎo)教師：周小華20091205重慶大學(xué)碩士學(xué)位論文英文摘要ABSTRACTThecross—lir山edcllitosaIlmicrosperemodifiedbyLysineisagoodca玎ieriniII】IIllobil幽genzymes，wIlichisnontoxic，physicalactiVe，d

2、e蓼adable，wIlilemodifedbyLysincmadeitmorefleXible，more緬Ilitive，wllichisa、ⅣonderMmemodCo—immobil婦enz”neisag乜lbleSystem，whichcallma：ke廿lereactioIlsc砌yzedbysin西e朗巧mejoiIledtogetllero瑪aIlically，rcalizetllesyne瑪isticactionb咖ee

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4、s—liIlkingagent，、歷mtheme廿lodofabsorptioncrosslinkTheopt砌zedcoIlditioIlsofprep幽gt11ec0一jmmbolizedGOD／cATare嬲follows：GOD／CAT=1／14Ⅳ／功moleratioofNH2be鉚ene11z]l，IIles：gl心砌dehyde：carrierisl：85：4，absorbingincoldw醣er，crossliIll【

5、inginr0鋤tcII驢r劬【u℃for3hoursThe鋤巧meactiV毋ofc0一iInmobilizeenzymeis153U／fmg，acti、ri哆recoVeryis671％1kmaillen巧meticpropertisesareaSfollows：Km23102I砌。兒，129缸esofthe毹eGoD’s，optimalpH40，optimaltempe怕ture42℃觚dthehalflifeforholding

6、at60℃，repeatedusetimesatroomtemperature(25℃)鋤dtorageat4℃is544ll，24tiIIlesand231daysspeci：ficlyThecoiIImlobilizee玎巧meis弱senlblized嬲C0lⅢmlbio—reaCo仃t0仃eatme舀uSo∞sohltioIl，theoptimalsubs慨econc即船aceis3％，flow豫：teis08m№i11Undc

7、rtheseconditions，thesta：blet血eforcontilliouslyoxidiziI唱me仔eshglutionSolutionis115h，recyliI瑪for4tiIIles，tllemaXim啪conVersionratec趾reacht045％Thercis∞h)1怕genperoxide(H202)acc啪ulatioIl，WIlichmeaIlsmedisadV觚_tagescausedbyH202

8、SuCh弱oXidizingGOD0rct啪咖gmesolutionpHhavebe鋤eleminated，als0itcanbeinfe硼tllatgluconateis也IeinKborUsing恤scol咖mtocontiIliouSlyhydroli∞t11e40timesdilutedXylitolmotherliquidcon詞幽g5％glucose，t：hema)【m哪conversi‘)nrateis352％Keywor