狹鱈魚皮膠原蛋白多（肽）制備與活性研究.pdf

1、分類號：Q959密級：UDC：579學(xué)校代碼：11065碩士學(xué)位論文狹鱈魚皮膠原蛋白多（肽）制備與活性研究狹鱈魚皮膠原蛋白多（肽）制備與活性研究孔令娟孔令娟指導(dǎo)教師王春波王春波教授教授韓彥弢韓彥弢副教授副教授學(xué)科專業(yè)名稱制藥工程論文提交日期2014年10月20日論文答辯日期2014、12、2答辯委員會主席AbstractObjective:Collagenpeptideswerederivedbyenzymatichydrolysis.

2、Themolecularweightseveralphysiochemicalpropertiesmicroganismcontentoffivederivedsamplesweredetermined.ThesafetyprotectivefunctionsagainstUVBwereinvestigatedfthefurtherclinicalapplication.Methods:ThecollagenpeptidesofPoll

3、ackskinwerehydrolyzedby5enzymes.Theproteiniccontentdeterminationwaterdeterminationashdeterminationmicroganismcontentdeterminationwereassayedbythenationalstardmethods.ThecontentdeterminationofhydroxyprolinewasperfmedbyUVS

4、pectrophotometry.TheprotectivefunctionsagainstUVBwerestudiedbyMTTmethod.SPSSsoftwarewasusedfthestatisticalanalysis.Results:Themajitymolecularweightdistributionofthederivedsampleswasfrom500to3000D.Thecontentsofproteinwate

5、rashmicrobewereallwithinnationalstards.Sample5#werediscoveredtoinhibittheHaCaTcelldamagebyUVB(30mJcm2)in100500μgmL.Nonethese5samplesshowedgrowthinhibityeffectsofHaCaTcellswithintheconcentrationof500μgmL.Conclusions:Thein

6、vestigationresultsrevealthatallsamplecollagenpeptidesderivedfromPollackskinisofsignificantmarketdevelopmentvalue.Thefoodproductionlicenseof5#samplehasbeenapproved(QS:370222022215).Keywds:skinsofPollackcollagenpeptidespre