版權(quán)說(shuō)明:本文檔由用戶提供并上傳,收益歸屬內(nèi)容提供方,若內(nèi)容存在侵權(quán),請(qǐng)進(jìn)行舉報(bào)或認(rèn)領(lǐng)
文檔簡(jiǎn)介
1、Food is the only source of life and it offers invaluable advantages: growth and health promotion, energy, and disease prevention; hence, life will not exist without food.Peoplelike enjoying sharing meal-gathering togethe
2、r and like to taste different kinds of traditionalcuisines; yet they also demand for consumption of quality and safe food.Principally, food,generally reconsidered as safe (GRAS), should be strictly produced from sanitati
3、ons, good manufacturing practices, good handling practices, and good agriculture practices toconform with food safety system guideline in order to prevent the contamination orrecontamination, especially microbial pathoge
4、ns and toxic chemicals, because these materials cannot be visually detected.Food contaminations resulting to food poisonings areobviously very serious issues to public health, living welfare, and socio-economicdevelopmen
5、t; therefore, they need to be tightly controlled (prevention, reduction, and elimination) before serving on the tables or markets.To tackle such these difficultproblems is a main task and huge challenge for regulatory au
6、thorities, public healthagencies, and food industries in order to ensure the quality and safe-to-eat food.
Because of visually impossible detection of food hazards, numerous sophisticallyefficient analytical methods
7、 have been successfully developed in order to meet the growing demands for food safety and quality control and supervision.Although food toxicity mostly comes from pathogenic microbes, toxic chemicals; such as pesticide
8、residues,illegal food additives, drug residues; are also a major threat to the life and public health;therefore, there is an increasingly considerable interest in developing new selective and sensitive method for extract
9、ing, isolating, and enriching contaminated components from complex food matrices.One of the promising methods that have been innovated so far isa molecularly imprinting technique, which has drawn a great attraction world
10、wide in thefield of chemical separation science.The exploitation of this technique could produce aproduct of molecularly imprinted polymers (MIPs); which are very robustness, long-termstability, reliability, cost-efficie
11、ncy, and selectivity; creating MIPs to gain more popularityin chemical separation and analysis.
In this dissertation, we would like to introduce "an analytical method" using MIPs-based materials as a solid phase ext
12、raction for selective recognition of given chemical molecules from the food matrices before high performance liquid chromato graphicde termination.The technique of MIPs synthesis is based on a traditional method, which b
13、ulk MIPs need to be ground, sieved, and Soxhlet washed prior to applying them as amolecularly-imprinted solid-phase extraction (MISPE) for sample clean-up and enrichment.The main functional monomer and cross-linker we us
14、e in MIPs polymerization is β(beta)-cyclodextrin, a 7-membered sugar ring molecule and ethylene glycol dimethacrylate(EGDMA), respectively.Our introduced method of MIPs polymerization is simple and inexpensive; moreover,
15、 the volume of organic solvent involved in the synthesis of MIPsand the rest of experimental process requires minimally, which is in parallel with environmentally-friendly way.Our findings are as follows.
(1)In chap
16、ter two, we prepared a series of di (2-ethylhexyl) phthalate (DEHP)imprinted polymers by using the single use of ailyl bromine-β-cyclodextrin (allyl-β-CD)and the combined use of allyl-β-CD and methacrylic acid (MAA), all
17、yl-β-CD and methylmethacrylate (MMA), allyl-β-CD and acrylonitrile (AN), and allyl-β-CD and acrylamide(AA) as the binary functional monomers.The results proved that the binary function almonomers, except for AA monomer,
18、are superior to a single monomer; their average bound substrate from binary monomers was ~110 μmol g-1, where as a single was ~90μmol g-1 in binding specificity.Finally, M-MAA, M-MMA and M-AN were chosen to runthrough mo
19、lecularly imprinted solid-phase extraction (MISPE) to analyze the spiked infantformula of DEHP.For M-AN, the recovery ranged from 93.59-97.98% with relativestandard deviations (RSD ≤ 3.21%).
(2)In chapter three, we
20、synthesized three kinds of clenbuterol-imprinted polymers bythe combined use of ally-β-cyclodextrin (ally-β-CD) and methacrylic acid (MAA), allyl-β-CD and acrylonitrile (AN), and allyl-β-CD and methyl methacrylate (MMA)
21、as the binaryfunctional monomers.Based upon the results, M-MAA polymers generally proved to be anexcellent selective extraction compared to its references: AN-linked allyl-β-CD MIPs (M-AN) and MMA-linked allyl-β-CD MIPs
22、(M-MMA).M-MAA polymers were eventuallychosen to run through a molecularly imprinted solid-phase extraction (MI-SPE) microcolumn to enrich CLEN residues spiked in pork livers.A high recovery was achievedranging from 91.03
23、-96.76% with relative standard deviation (RSD ≤ 4.45%).
(3)In chapter four, we exploited binary functional monomers, allyl-β-cyclodextrin(allyl-β-CD) and methacrylic acid (MAA) or allyl-β-CD and acrylonitrile (AN),
24、in afabrication of molecularly imprinted polymers (MIPs) for selective recognition and largeenrichment of pirimicarb pesticide from aqueous media.According to the results, the effectof binding capacity of MAA-linked ally
25、l-β-CD MIPs (M-MAA) demonstrated higherefficiency than that of AN-linked allyl-β-CD MIPs (M-AN) when tested in bindingspecificity.Finally, M-MAA was chosen to run through molecularly imprinted solid-phaseextraction (MISP
26、E) to analyze the spiked fresh leafy vegetables of pirimicarb.The presentproposed technique is a promising tool for the preparation of the receptors which couldrecognize pirimicarb pesticide in aqueous media.
Accord
27、ing to the aforementioned results, we found that our synthesized MIPs could beused for a sample clean-up and pre-concentration of target analytes of interest in foodmatrices.Our MIPs showed good stability, good selectivi
28、ty, and high efficient adsorptioncapacity towards the target molecules; therefore, they could be applied to real food samplesas an integral part of analytical method for food quality and safety control and supervision.
29、r> There are a variety of applications of MIPs such as off-line or on-line solid phaseextraction (SPE), chemical and bio-sensors, catalysis, and drug delivery because MIPs, asynthetically potential artificial receptor-l
30、ike binding sites with a "memory" for shape andfunctional group positions of the target molecule, possess a competent ability for selectivespecificity and recognition for target or unwanted chemical molecules.Among vario
31、usMIPs applications, the most commonly used is an off-line solid phase extractionapplication due to its simplicity.Utilizations of MIPs-based materials have been applied toa wide range of chemicals, including food contam
32、inants, pesticides, environmentalpollutants, preservatives, and antibiotic drug residues for sample clean-up and preconcentration, detection, and quantification.
All in all, due to various functionalities of MIPs su
33、ch as solid phase extraction (SPE),chemical and bio-sensors, catalysis, and drug delivery, molecular imprinting technique hasdrawn a huge attraction from a wide range of fields, including food safety, chemistry,biology,
溫馨提示
- 1. 本站所有資源如無(wú)特殊說(shuō)明,都需要本地電腦安裝OFFICE2007和PDF閱讀器。圖紙軟件為CAD,CAXA,PROE,UG,SolidWorks等.壓縮文件請(qǐng)下載最新的WinRAR軟件解壓。
- 2. 本站的文檔不包含任何第三方提供的附件圖紙等,如果需要附件,請(qǐng)聯(lián)系上傳者。文件的所有權(quán)益歸上傳用戶所有。
- 3. 本站RAR壓縮包中若帶圖紙,網(wǎng)頁(yè)內(nèi)容里面會(huì)有圖紙預(yù)覽,若沒(méi)有圖紙預(yù)覽就沒(méi)有圖紙。
- 4. 未經(jīng)權(quán)益所有人同意不得將文件中的內(nèi)容挪作商業(yè)或盈利用途。
- 5. 眾賞文庫(kù)僅提供信息存儲(chǔ)空間,僅對(duì)用戶上傳內(nèi)容的表現(xiàn)方式做保護(hù)處理,對(duì)用戶上傳分享的文檔內(nèi)容本身不做任何修改或編輯,并不能對(duì)任何下載內(nèi)容負(fù)責(zé)。
- 6. 下載文件中如有侵權(quán)或不適當(dāng)內(nèi)容,請(qǐng)與我們聯(lián)系,我們立即糾正。
- 7. 本站不保證下載資源的準(zhǔn)確性、安全性和完整性, 同時(shí)也不承擔(dān)用戶因使用這些下載資源對(duì)自己和他人造成任何形式的傷害或損失。
最新文檔
- 用于食品安全檢測(cè)的分子印跡聚合物計(jì)算模擬研究.pdf
- 谷胱甘肽的分子印跡聚合物制備及其吸附選擇性規(guī)律研究.pdf
- 分子印跡聚合物膜的制備、滲透選擇性識(shí)別機(jī)理及其應(yīng)用研究.pdf
- 以殼聚糖為載體新型免疫親和吸附劑及分子印跡聚合物的制備與應(yīng)用研究.pdf
- 21211.β環(huán)糊精星型聚合物的刺激響應(yīng)性研究及β環(huán)糊精聚合物刷作為多功能基因載體的研究
- 通過(guò)分子印跡聚合物改性松香交聯(lián)劑選擇性吸附延胡索乙素外文翻譯
- 桿菌-酵母菌表面分子印跡聚合物的制備及其選擇性吸附-分離環(huán)丙沙星的研究.pdf
- 畢業(yè)論文——通過(guò)分子印跡聚合物改性松香交聯(lián)劑選擇性吸附延胡索乙素
- β-環(huán)糊精高分子聚合物的制備及其應(yīng)用.pdf
- 具有重要藥用價(jià)值化合物的分子印跡聚合物制備及其吸附選擇性規(guī)律研究.pdf
- 通過(guò)分子印跡聚合物改性松香交聯(lián)劑選擇性吸附延胡索乙素外文翻譯.doc
- 磁性分子印跡聚合物選擇性吸附水相中磺胺二甲嘧啶的研究.pdf
- 氮?dú)膺x擇性吸附劑研究.pdf
- 高嶺土及其改性材料表面印跡聚合物選擇性吸附雙酚A.pdf
- 4968.核酸探針與環(huán)糊精聚合物用于生物分子熒光檢測(cè)的研究
- 10460.功能性β環(huán)糊精聚合物固相萃取分離分析應(yīng)用研究
- 分子印跡聚合物在環(huán)境污染物殘留分析及吸附中的應(yīng)用研究.pdf
- 煙堿分子印跡聚合物的識(shí)別機(jī)理與分析應(yīng)用研究.pdf
- 分子印跡聚合物及其在分離分析中的應(yīng)用研究.pdf
- 分子印跡聚合物的合成、識(shí)別機(jī)理及分析應(yīng)用研究.pdf
評(píng)論
0/150
提交評(píng)論