呼和浩特市5種針葉樹內(nèi)生真菌的研究.pdf

1、分類號S763.19學(xué)校代碼10129UDC630學(xué)號2012203011呼和浩特市5種針葉樹內(nèi)生真菌的研究TheStudyofEndophytein5SpeciesofConiferousinHohhot申請人：烏日罕學(xué)科門類：農(nóng)學(xué)學(xué)科專業(yè)：森林培育研究方向：林木菌根生物技術(shù)指導(dǎo)教師：閆偉教授論文提交日期：二〇一五年六月TheStudyofEndophytein5SpeciesofConiferousinHohhotAbstractI

2、ndertoexpletheconiferousendophyticfungicompositionstructuredifferencescolonizationsiteofconiferous.CleartheresourcestatusofconiferousendophyticfungiinHohhot.ThepaperinvestigatedconiferousfromfourdifferentplantingsiteinHo

3、hhotbytissueisolationmethodtoidentifiedendophyticfungi，analysedthebiologicalacteristicsofthedominantgenera.Theresultsshowthat:1.1636strains(209strainsofnonspe)ofendophyticfungiareseparatedfromfivespeciesofconifersbelongi

4、ngto2classes5ders6families35genera.303strainsofendophyticfungiareseparatedfromJuniperusregidaSieb，etZuccbelongingto17genera314strainsofendophyticfungiareseparatedfromPinustabulaefmisCarr.belongingto17genera384strainsofen

5、dophyticfungiareseparatedfromSabinachinensisbelongto17genera326strainsofendophyticfungiareseparatedfromPiceaasperataMast.belongto13genera309strainsofendophyticfungiareseparatedfromPinussylvestrisLinn.var.mongolicaLitv.be

6、longingto16genera.Alternariasp.Penicilliumsp.arethepredominantgrneraoffiveconiferouswith27.81%13.14%ofthegrossrespectively.2.386strainsofendophyticfungiareseparatedfromQingChengParkbelongingto18generaThelargestnumberofth

7、estrainsthegenera.Differentplantingsitesofconifersendophyticfungihavedifferentpopulationstructures.Insummaryendophyticfungihaveacertainrelationshipwithplantingaffestationenvironment.3.361strainsofendophyticfungiaresepara

8、tedfromtheneedlesbelongingto22generaThelargestnumberofthestrainsthegenera.Penicilliumsp.isthedominantgenerainconifersiteswith4.09%ofthegrossrespectively.Someendophyticfungionlyseparatedfromthesamepartthatisshowthatendoph

9、yticfungihavetissuespecificity.4.Intheanalysisofbiologicalacteristics25℃isthebestgrowthofdifferenttemperatureconditionsofAlternariasp.Penicilliumsp..GlucoseistheoptimalcarbonsourceofAlternariasp.sucroseistheoptimalcarbon

10、sourceofPenicilliumsp.AmmoniumnitrateasisthemainnitrogensourceofAlternariasp.potassiumnitrateisthemainnitrogensourceofPenicilliumsp..Thegrouthofendophyticfungiwereincreasedwithconcentrationsugarconcentration.KeyWds:conif