相思樹的細胞培養(yǎng)及體胚發(fā)生研究.pdf

1、福建農(nóng)林大學(xué)碩士學(xué)位論文相思樹的細胞培養(yǎng)及體胚發(fā)生研究姓名：葉玲娟申請學(xué)位級別：碩士專業(yè)：園林植物與觀賞園藝指導(dǎo)教師：賴鐘雄20080401福建農(nóng)林大學(xué)碩士學(xué)位論文摘要StudiesoncellcultureandsomaticembryogenesisinAcaciasppAbstractThecallifromAcaciacD，!血陽，AcaciaconcinnataandAcaciamelanoxylonwereusedasini

2、tialmaterialsinthisexperimentwhichincludedtheeffectsofdifferentcultureconditionsonmaintainingandregulatingthephysiologicalconditionsofealli，thecomparisonofthedifferencesamongthethreekindsofAcaciaintheestablishmentandmain

3、tenanceofcellsuspensionculture，andtheseparationofsinglecellofthethreespeciesofAcaciafromsuspensioncellculture，andestablishmentofthecloningsystemofsinglecellsMeanwhile，thecalliofAcaciamelanoxylonwereusedtoestablishthesyst

4、emofsomaticembryogenesisofAcaciamelanoxylon，andcytologicalobservationduringsomaticembryogenesisandcomparisonofthechangesofperoxidaseactivitiesandproteincontentsduringsomaticembryogenesiswerealsocarriedoutThemainresultswe

5、reasfollows：1EstablishmentofmaintenancesystemforthecallusThedifferenteffectssuchasplantgrowthregulators，lightconditions，andgenotypesonthemaintenanceofthethreespeciesofAcaciawerecomparedintheexperimentandtheresultsshowedt

6、hatMSmediumsupplementedwith02mgL～24D，O5IngL1BA，and02mgL。1NAAWasbeneficialtothemaintenanceofcalli，andthesuperiorinoculationamountwas03gealliineachbottle2StatusregulatingandscreeningofAcaciacallusTheeffectsofdifferent2，4一D

7、concentrationsonthestatusofAcaciacalliwereanalyzedinthisexperimentandtheresultsshowedthatthecallicouldbewellmaintainedonthemediumsupplementedwith02一lmgr12，4D，andmostofthecalliwerefreshyellowbelongingtothelooseandsoftcall

8、iIandII，whichweresuitableforcellsuspensioncultureMoreovertheeallicouldbeIceptlooseandsoftintheweaklightbycomparingtheeffectsofdifferentlightconditions3ThemaintenanceofcellsuspensionsystemsforthreespeciesofAcaciasppTheeff

9、ectsofdifferentgenotypes，callustypes，concentrationsof2，4D，sucroseconcentrations，andinoculationamountonthecellsuspensionculturewereanalyzedintheexperimentandtheresultsshowedthatthebestinitialmaterialoftheAcaciasppforcells

10、uspensioncultureWasthe丘eshyellowlooseandsoftcallusIandII，whichwerepropitioustoestablishthesuspensioncellculturesystemquicklyInadditionthecellsoftheAcaciamelanoxylonweresmall，ofstrongernbryogeniccharacteristicsandfinedisp

11、ersionwhichwereeasytoestablishthecellsuspensionsystemThecellsuspensioncouldbemaintainedwellundertheconditionsthatthesucroseconcentrationWas30g／Landthe2，4DconcentrationWas02mg／LAndtheinitialinoculationamountalsoinfluenced