固氮芽孢桿菌的分離鑒定以及固氮巨大芽孢桿菌（Bacillus megaterium）C4在玉米、水稻、小麥上的定殖研究.pdf

1、中國農(nóng)業(yè)大學(xué)碩士學(xué)位論文固氮芽孢桿菌的分離鑒定以及固氮巨大芽孢桿菌（Bacillusmegaterium）C4在玉米、水稻、小麥上的定殖研究姓名：劉旭明申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：陳三鳳20050601AbstractBacillusmegateriumC4，anitrogenfixingbacterium，wasmarkedwith咖geoeMaize，riceandwheatseedlingswereinoculate

2、dwiththeGFPlabeled且megateriumC4andthengrowningnotobioticcondition，Themaizeroots，stems，leaves，decfoolsandwheatrootswereopticallysectionedandobservedunderconfoeallaserscanningmicroscopeObservationsofthemakerootsectionsat1，

3、3，5，7，9and11daysafterinoculationshowedsignificantlytheinfectionprocessthatthebacterialcellsinfectedthemalzerootsfromtherootsurfacestothestelesOnedayafterinoculation，thebacteriacolonizedmainlythe汕rfacesofprimaryandlateral

4、roots，andthejunctionsbetweenprimaryrootandlateralrootThreedaysafIcrinoculation，thebacterialcellscolonizedcortexesFivedaysafterinoculation，thebacteriahadprogressedtowardsinnercortexSevendaysafterinoculation，thebacteriarea

5、chedvascularsteleandpenetratedintoxylemandpith1kbacteria、Ⅳ盯emainlylocatedintheintercellularspaecsalthoughafewbactexialcellsalsoⅥ℃mpresentwithinthexylemvesselsroothair∞ns，epidermis，corticalparenchymaandpithcellsAprobables

6、iteofeutryintomaizerootsforthebacteriawascracksformedatthelateralrootjunctionsMicroscopicobservationsofthesectionsofmaizestemsandleavesat30and40daysafterinoculationrevealedthatthebacteriawerepresentinstemsandleavesat30an

7、d40daysaftelinoculation，indicatingthatthebacteriamigratedslowlyfromtootstostemsandleavesColonizationstudiesofricelOOtSandwheatrootsat1，3，5，7daysafterinoculationrevealeddearlytheinfectionprocessonriecrootsandwheatrootswas

8、similartothatonmaizeroots，andthebacterialcellswerefoundtocolonizeepidermiscortexandvasculartissuesofricerootandwheatrootItalsoindicatedthatthemottip蛔thezoneofelongationandthejunctionsbetweentheprimaryandthelateralrootswe

9、rethetwositesforthebacteriaenuyinticerootThepopulationofthebacterialcellscolonized抽themaizerootsstemsandleavesWeTgestimatedbycountingcolonyformingunitsonselectivemedium11他populationinrootsreached1礦。cFu／gFWat3daysafterino

10、culationandgraduallyincreasedto10’。CFU／gFWat9daysafIerinoculationAt30daysafterinoculationthebacterialpopulationreached10&2CFU／gFWinleavesand104‘3cexvgFWinstems。Soilsamplesofdiffe犯utplantrhlzosphe∞werecollectedkomfifteenp

11、rovincesThe!／vcetemadeintosuspensionsandseethedfor15aria，thenplatedonnitrogenfleemediuminordertoisolatenitrogen—fixingbacillusColonyPCRw∞usedtoamplifyn／／Hgenesegmentandnitrogenaseactivitywasdeterminedbyacetyleeereduction

12、method116strainsofnitrogenfLxingbacilluswhichbo也have^d曰geaesegmentandnitrogenaseactivitywereacquired，andnitrogenaseactivitiesof7strainsexceededthatoftypestrainPazotoflxonsDSMl735Keywords：BacillusmegateriumCA；GFPlabcled；c