食品中某些有害物質(zhì)的GC-MS和高效毛細(xì)管電泳檢測新方法.pdf

1、河北大學(xué)碩士學(xué)位論文食品中某些有害物質(zhì)的GCMS和高效毛細(xì)管電泳檢測新方法姓名：霍利申請學(xué)位級別：碩士專業(yè)：分析化學(xué)指導(dǎo)教師：呂運開20100501AbstractIIAbstractInrecentyearsthechromatographydevelopsrapidlyithasbeenusedwidely.Inthepapertheapplicationofchromatographyinfoodsafetyanalysishas

2、beenreviewedincludingapplicationofGCMSHPCEinfoodanalysis.Inchapter2anovelmethodofhighperfmancecapillaryelectrophesis(HPCE)hasbeenestablishedftheseparationofmelamineinmilkmilkpowder.Trichloaceticacidsolutionwasusedtopreci

3、pitateproteinstodissociatethemelaminefromthesamplematrix.Indertodissociatetheproteinscleansimplythesamplewasheatedtoboilthencoolednaturally.Theaveragerecoveriesrangedfrom83.2%to97.5%inthespikedrangeofthedeterminationofme

4、lamineinmilkmilkpowder.Thelimitofquantification(LOQs)ofmilkmilkpowderwas1.0mgkgtherelativestarddeviationswerebetween2.1%3.9%(n=5).Thesensitivityofthemethodissimilartothehighperfmanceliquidchromatographythesamplepretreatm

5、entismesimplespecificreproducibleeconomicenvironment.Soitcanbeusedfalargenumberofsamplesofmilkmilkpowder’smelaminerapiddetection.Inchapter3anewmethodofthedeterminationofdifenoconazoleinVegetablebyGCMSMSwasdeveloped.Thedi

6、fenoconazoleinsamplewasextractedwithacetonitrile.TheextractwaspurifiedconcentratedwithsolidphaseextractioncolumnthendetectedbyGCMSMSinthemultiplereactionmonitingmode.Theaveragerecoveriesrangedfrom78.82%to94.64%Thelimitof

7、quantification(LOQs)was10gkgThecalibrationcurvewaslinearbetween0.01mgL0.25mgL(10gkg250gkg)thecrelationcoefficientwasmethan0.99.Inchapter4anovelmethodofsimultaneousdifferminationofamitrazchldimefminhoneybyGCMSMSwasinvesti

8、gated.TheamitrazchldimefminsamplewereextractedwithmixtureofacetonenhexanetheextractwaspurifiedconcentratedthendetectedbyGCMSMS.Thecalibrationcurvewaslinearofamitrazchldimefm24xylidinebetween0.01mgL0.2mgL(10gkg200gkg)thec