轉(zhuǎn)Bt基因玉米秸稈對微生物及發(fā)酵效果影響的研究.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文轉(zhuǎn)Bt基因玉米秸稈對微生物及發(fā)酵效果影響的研究姓名：孟瑤申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：徐鳳花20080620，Abstract’’‘’1EffectofBtTransgenicCornStrawFermentationonMicrobeCommunityAbstractWiththetransgenicBtcropsplantedextensivelyinproduction，Btproteinwil

2、lenterthesoilthroughthedifferentwaysandaccumulatecontinuouslyThefollowingquestionisthatwhethertheseBttoxinwillhaveadisadvantageousinfluenceontheenvironmentmicroorganismButenvironmentaImicroorganismhaveconnectionwiththele

3、velofsoilfertility，productivityanditsabilityofsustainableuseSomuchattentionwasfocusedtotheenvironmentbehaviorandtheeffect0fBttoxinThelactobacilluscommunitySFC2andthecellulosedegradationfermentationcommunityMC1wereusedtos

4、trawfermentationintransgenicandnontransgeniccomThechangeofpH，thespeedofcellulosedecomposing，thequalitativeandquantitativeanalysisofdecomposingoutcome(GCMS)，thecontentofBttoxinweresurveyedentirelyDGGE(degenerationgradgele

5、lectrophoresis)methodprovideatestinmoleculelevelmicroorganismgeneticmaterialintheprocessoffermentationBycomparingthedifferencebetweenthetransgeniecornandnontransgeniccorn，theeffectwillbedescribedThetheorywillbeestablishe

6、dunderthestudyoftransgenicBtcorn(1)ThelactobacilluscommunitySFC一2wasinoculatedintothecoinstrawtoinvestigatethefermentationeffectspHvalueoftransgenicandnon—transgeniccornachievetherequestThesmelloffragranceisgoodThequalit

7、yofmaterialislooseandthelusterisbrightIntheinitialperiod，pHvaluedropsquicklyandreachtheminimumvalueatthe7thday，thenincreasesslowlyThereisnogreatsignificantdifferencebetweentransgenicandnontransgenicCOITISolublesugarconte

8、ntshowsthesametrendwithpHvalue，whichreachesthelowestvalueinthel3thdayThereiSalsonogreatsignificantdifferenceExceptthecrudeproteincontentincreasesalittle，thecontentofcrudefatandashdonotchangeduringthefermentationtimeAlsot

9、hedifferencebetweenthesecharacteristicsiSnotremarkableThemeasurementofBttoxincontentshowedthattwotransgenicCOindescendedsignificantlyinthe7thBttoxincontentreducedgraduallyduringthewholefermentationtimeGCMSshowedthatthere

10、iSnogreatsignificanceinthesefourcorn，suchasthecontentofethanol，aceticacid，lacticacidandglycerin(2)ThecellulosedegradationfermentationcommunityMClWasinoculatedintotheCOinstrawtoinvestigatethefermentationeffectsAmongtheset

11、ransgenieandnon—transgenieCOin，thereisn0significanceinpHvalue，thecontentofsolublesugar，thedryweightofstraw，thecontentofcelluloseandthecontentofNDFandADFGCMSshowedthatthereisnogreatsignificanceinthesefourcoin，suchasthecon

12、tentofmethylalcohoI，ethanol，aceticacid，IacticacidandvalericacidNoBttoxinWaSmeasuredinthefermentationfluidoftwotransgenicCOin，whichmaybedecomposedbytheMCIfermentation(3)DGGE(degenerationgradgelelectrophoresis)showedthatSF