基于量子點的離子-生物探針及一釜法制備硅殼型CdTe量子點的研究.pdf

1、湖南大學(xué)碩士學(xué)位論文基于量子點的離子生物探針及一釜法制備硅殼型CdTe量子點的研究姓名：王益林申請學(xué)位級別：碩士專業(yè)：分析化學(xué)指導(dǎo)教師：王柯敏20050701基于量子點的離子／生物探針及一釜法制備硅殼型CdTe量子點的研究AbstractThisthesisdescribestheuniqueopticalpropertiesofquantumdots(qdots)，currentapproachesforthesynthesisofq

2、dotsandtheirapplicationsonlifescienceresearchCdTeqdotsweresynthesizeddirectlyinaqueoussolutionbyusingthioglycolicacid(TGA)orcysteamine(MA)asthestabilizerBasedonTGAmodifiedqdots，anewapproachwasdevelopedforsensitiveandsele

3、ctivedeterminationofcopperionsInordertoimprovethestabilityofMA—modifiedqdotssilicalayerwasaddedtoeachindividualquantumdotFurthermorethesilica—coatedqdotsweresuccessfullyusedtothecellslabellingMeanwhile，anewonepotapproach

4、wasproposedforthefirsttimetopreparesilica—coatedqdots(1)CdTeqdotsweresynthesizedinaqueoussolutionbyusingTGAasthestabilizerTheywerecharacterizedbyUVVis，fluorescencespectroscopyandAFM，respectivelyWiththiskindofwater—solubl

5、eqdotsasfiuorescenceprobes，anovelapproachwasdevelopedforsensitiveandselectivedeterminationofcopperionsDifferentinfluencefactorswerestudied，includingthebufferconcentration，qdotsconcentration，pHvaluesandreactiontimeUnderth

6、eoptimizedconditions，therelativefluorescenceintensitydecreasedlinearlywiththecopperionsconcentrationintherangefrom2to200肛g’L“andthedetectionlimitcouldreach029pgL～Basedonthecharge—transferquenchingmechanism，thecopperionsb

7、oundingontothesurfaceofqdotscouldabsorbelectronsfromvalanceband，whichresultsinthequenchingoffluorescenceThiswasprovedbyphotolysisexperiment(2)TheMAcappedCdTeqdotswerepreparedinaqueoussolution，andthencharacterizedbyUVVisa

8、ndfluorescencespectroscopyInordertoimprovethestabilityofthiskindofqdots，athinsilicalayerwascovalentlyboundtothesurfaceofeveryparticletopreparesilica—coatedqdotsAmineandphosphonategroupsweremodifiedontheirsurfacesimultane

9、ouslyTheqdotsaftercoatingwasmonodisperseandwellseparatedVariousreactionconditionsthataffectedthepropertiesofsilica—coatedqdotswerestudied，includingligandexchangetime，thedosageofTEOSandthereactiontemperatureFurthermore，th

10、esilica—coatedqdotswerecharacterizedbyaseriesoftechniques，suchasfluorescencespectra，AFM，sizedistributions，Zetapotentialandphotobleaching，etcMoreover，itwassuccessfullylinkedwithlactobionicacidthroughaminegroupsonthesurfac