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1、福建農(nóng)林大學(xué)碩士學(xué)位論文60Coγ射線輻射5個(gè)決明牧草品種M1M5的遺傳變異研究姓名:鄭向麗申請(qǐng)學(xué)位級(jí)別:碩士專業(yè):生物工程指導(dǎo)教師:祁建民;徐國(guó)忠200710福建農(nóng)林大學(xué)丁程專業(yè)碩士學(xué)位論文Abstractinthisstudytheseedsof5leguminousforagesinChamaecristawereradiatedby5dosagesof60Co1/rayinordertofindanappropriateradi

2、atingdoseThentheappropriatedoseforeachvarietywasadoptedintheradiationbreeding,andtheagronomicandqualitypropertiesofMlM5infivehereditarygenerationswereobservedScreeningofthedescendantsfromradiationmutantshasbeenundertaken

3、inbothpotcultureandfieldconditionThepotcultureWasalsousedtoobserveandanalyzethevariationfromradiationtreatmentIntheexperiments,TheseedsinMjgenerationafterradiationwereharvestedinmixturecondition;theseedsinM2werecollected

4、fromthesingleplantwhichshowedthevariation;themutantstrainsweretestedinitsstabilityinM3generationBasedtheexperimentalpurposeandappearanceofthemutantsinM3,SixmutantdescendantsfromstabilizedstrainsinM3generationwereselected

5、toconducttheplotcomparisoninfieldconditioninM4andM5generationsTheRAPDanalysistogenomeDNAfromsomestrainswasundertookTheexperimentalresultswereasfollows:1Usinglinearregressionequation,theappropriatedosagesfortheradiativein

6、ductionofstrains22I7,2219,34721,86134and92985were527Gy665GB695Gy’523Gyand598Gyrespectively2VariabilityofagronomicpropertyinM1一M2generationsafterradiation:ThereproductivegrowthperiodoffivestrainsinCharaaeeristashowedabidi

7、rectionalvariationaftertheradiativeinduction,ieboththevariabilitieswithshorteningorpostponingthereproductivegrowthperiodwerefoundThecrownheightof34721,thestemdiameterof2219WaSsignificantlyincreasedthebranchnumberof86134W

8、aSsignificantlyincreasedbutthatof92985wassignificantlydecreased,theleafnumberof86134,theleaflengthof2219WaSsignificantlyincreased;nosignificantdifferenceWasfoundinotheragronomicpropertiesThetesttonutritionalcontentfN,PK)

9、inplantshowedthat,totalKin2219Wassignificantlydecreased,theothershavenosignificantdifferenceRAPDanalysisonthestrainsinM2showedthatthereWassignificantdifferenceinDNAbandsbetweencontrolandradiationdescendantsItmeansthegene

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