PTCH在胃粘膜腸上皮化生、不典型增生和胃癌中的表達.pdf

1、山西醫(yī)科大學碩士學位論文PTCH在胃粘膜腸上皮化生、不典型增生和胃癌中的表達姓名：武偉申請學位級別：碩士專業(yè)：外科學指導教師：蘇繼榮閻慧明20080313坐墮墾型奎堂要主蘭垡絲苧ThePTCHexpressioninintestinalmetaplasia、atypicalhyperplasiaandcarcinomaofgastricmucosaAbstractObjectiveToinvestigatetherelationship

2、ofPTCHinHedgehogsignaltransductionpathwayandthedifferentialstage，invasion，clinicalstage，caregogensisofgastriccarcinoma。MethodsnleexpressionofPTCHproteinumWasevaluatedbyimmunohistochemistryin42casesofgastriccancertissues、

3、15casesofatypicalhyperplasiatissues、20casesofintestinalmetaplasiatissues、and7casesofnormalgastrictissues。TocomparetheexpressionofPTCHproteinuminatypicalhyperplasiatissues、normalgastrictissues、intestinalmetaplasiatissuesa

4、ndgastriccancertissuesAndsimultaneously，detectingandsemiquantitativingtheexpressionofPTCHmRNAbyRtPCRin42casesofgastriccancertissues、15casesofatypicalhyperplasiatissues、20casesofintestinalmetaplasiatissues、and7casesofnorm

5、algastrictissuestoinvestigatetherelationshipofPTCHandcaregogensisofgastriccarcinomaInadditionwecomparedtheexpressionofPTCHwithclinicopathologiccharacteristicsofgastriccancerResult①neexpressionsofPTCHgeneinnormaltissues、i

6、ntestinalmetaplasiatissues、atypicalhypcrplasiatissuesandgastriccancertissuesweredifferenceandincreased、^ritllstaging(PO05)PTCHgenewasnotdetectedinnormaltissues；Inintestinalmetaplasiatissues，theexpressionofPTCHproteinumwe

7、remascclinein2pousofintestinalmetaplasiatissues(10％)，thedyeingintensitywere()，theoppositiveexpressionofPTCHmRNAWasO07004；InatypicalhyperplasiatissuestheexpressionofPTCHproteinumweremascclinein6pous(40％)，amongthetotal，the

8、dyeingintensitywere()in2pousand)in4pous，theoppositiveexpressionofPTCHmRNAWas01801O；Ingastriccancertissues，theexpressionofPTCHproteinumweremascclinein33pous(786％)andthedyeingintensitywere(卅)and(抖抖)in19pous，theoppositiveex

9、pressionofPTCHmRNAwas062_011②1f11eexpressionofPTCHproteiningastriccancertissueswascorrelatedcloselywithdifferentiateddegree、infestationandclinicalstageofgastriccancerConclusion(DPTCHwasrelatedwithdifferentiateddegree、inf