2023年全國(guó)碩士研究生考試考研英語(yǔ)一試題真題(含答案詳解+作文范文)_第1頁(yè)
已閱讀1頁(yè),還剩55頁(yè)未讀, 繼續(xù)免費(fèi)閱讀

下載本文檔

版權(quán)說(shuō)明:本文檔由用戶(hù)提供并上傳,收益歸屬內(nèi)容提供方,若內(nèi)容存在侵權(quán),請(qǐng)進(jìn)行舉報(bào)或認(rèn)領(lǐng)

文檔簡(jiǎn)介

1、 分類(lèi)號(hào):R739.4 密級(jí):不保密 UDC UDC:610 學(xué)校代碼:11065 碩 士 學(xué) 位 論 文 2,3-吲哚醌抗人神經(jīng)母細(xì)胞瘤 吲哚醌抗人神經(jīng)母細(xì)胞瘤 SH SH-SY5Y SY5Y 細(xì)胞侵襲及遷移機(jī)制研究 細(xì)胞侵襲及遷移機(jī)制研究 徐平平 指 導(dǎo) 教 師侯琳

2、 教授 學(xué) 科 專(zhuān) 業(yè) 名 稱(chēng) 生物化學(xué)與分子生物學(xué) 論 文 提 交 日 期 2 0 1 5 年 5 月 1 1 日 論 文 答 辯 日 期 2 0 1 5 年 6 月 3 日 答辯委員會(huì)主席 劉均洪 教授 Abstract Background: Isatin is widely present endogenously in both human and other mammalian tissues and

3、 fluids likely as a result of the tryptophan metabolic pathway. Isatin showed significant inhibiting effect on the growth of SH-SY5Y neuroblastoma cells. The objective of this study was to investigate that isatin is also

4、 able to alter the invasion and migration ability of SH-SY5Y cells and probably mechanism. Methods: SH-SY5Y cells were exposed to isatin at various concentrations (100, 200 and 400 μmol/L). Invasion assays were conducted

5、 to detect the invasiveness and scratch assay was used to analye metastasis. MMP2 and MMP9 mRNA was analyzed by Real Time RT-PCR. MMP2, MMP9 and pSTAT3 protein were analyzed by Western blotting. Results: Isatin caused th

6、e most significant inhibition of cell migration and invasion. The inhibition rates of invaded cells were 42.14 % and 87.42 %, respectively (P<0.01). Wound-healing assay showed that cell migration was reduced in 200 μm

7、ol/L group compared with the control group at 36 h and 48 h, respectively. The inhibition rates of migrated cells were 42.78 % and 45.03 %, respectively (P<0.05). Treated with isatin, the expression levels of MMP2 and

8、 MMP9 mRNA and protein was decreased (P<0.05). Moreover, phosphorylated STAT3 was decreased in SH-SY5Y cells with isatin in a concentration-dependent manner (P<0.01). Conclusions: Isatin can inhibit migration and i

9、nvasiveness probably by reducing MMP2 and MMP9. The reason leading to these alterations might be that isatin down-regulates the level of pSTAT3. Postgraduate student: Xu Pingping (Biochemistry and Molecular Biology) Dire

溫馨提示

  • 1. 本站所有資源如無(wú)特殊說(shuō)明,都需要本地電腦安裝OFFICE2007和PDF閱讀器。圖紙軟件為CAD,CAXA,PROE,UG,SolidWorks等.壓縮文件請(qǐng)下載最新的WinRAR軟件解壓。
  • 2. 本站的文檔不包含任何第三方提供的附件圖紙等,如果需要附件,請(qǐng)聯(lián)系上傳者。文件的所有權(quán)益歸上傳用戶(hù)所有。
  • 3. 本站RAR壓縮包中若帶圖紙,網(wǎng)頁(yè)內(nèi)容里面會(huì)有圖紙預(yù)覽,若沒(méi)有圖紙預(yù)覽就沒(méi)有圖紙。
  • 4. 未經(jīng)權(quán)益所有人同意不得將文件中的內(nèi)容挪作商業(yè)或盈利用途。
  • 5. 眾賞文庫(kù)僅提供信息存儲(chǔ)空間,僅對(duì)用戶(hù)上傳內(nèi)容的表現(xiàn)方式做保護(hù)處理,對(duì)用戶(hù)上傳分享的文檔內(nèi)容本身不做任何修改或編輯,并不能對(duì)任何下載內(nèi)容負(fù)責(zé)。
  • 6. 下載文件中如有侵權(quán)或不適當(dāng)內(nèi)容,請(qǐng)與我們聯(lián)系,我們立即糾正。
  • 7. 本站不保證下載資源的準(zhǔn)確性、安全性和完整性, 同時(shí)也不承擔(dān)用戶(hù)因使用這些下載資源對(duì)自己和他人造成任何形式的傷害或損失。

最新文檔

評(píng)論

0/150

提交評(píng)論